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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">We’ll put it in our -20.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Cheers,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D">Bruce<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif"> Jason Zhu <jasonz13@uci.edu>
<br>
<b>Sent:</b> Wednesday, July 19, 2023 9:44 AM<br>
<b>To:</b> Bowler, Bruce <Bruce.Bowler@mso.umt.edu><br>
<b>Cc:</b> Borries Demeler <demeler@gmail.com>; James S. Nowick <jsnowick@uci.edu>; Henrickson, Amy <amy.henrickson@uleth.ca>; demelerlab@biophysics.uleth.ca; maduni.ranasinghe@uleth.ca; Frederick, Ariel <ariel.frederick@umconnect.umt.edu><br>
<b>Subject:</b> Re: AUC Collaboration: Sizing Amyloid Beta derived Oligomers<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Hi Bruce and Ariel,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">A lower storage temperature (such as -20 <sup><span lang="FR" style="font-size:8.5pt;font-family:"Arial",sans-serif;color:#202122">o</span></sup>C) would be better however 4<sup><span lang="FR" style="font-size:8.5pt;font-family:"Arial",sans-serif;color:#202122">o</span></sup>C
should also work. Thanks for keeping an eye out for the shipment, I really appreciate it!<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Best, <o:p></o:p></p>
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<p class="MsoNormal">Jason<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Wed, Jul 19, 2023 at 7:02 AM Bowler, Bruce <<a href="mailto:Bruce.Bowler@mso.umt.edu">Bruce.Bowler@mso.umt.edu</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">Hi Borries and Jason,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">We'll keep an eye out for your shipment. I take it you want it stored at 4 oC when it arrives. Let me know if storage at a lower temperature is better.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">For pick up on Friday, sometime between 10:30 am and 2:30 pm is best for me.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Bruce<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Sent from my T-Mobile 5G Device<br>
Get <a href="https://urldefense.com/v3/__https://aka.ms/AAb9ysg__;!!CzAuKJ42GuquVTTmVmPViYEvSg!MMksFEt9h3wTNKbwVqKh2ZNZIsCFG0cn6YYIfTtveN0_nfAC7g4LrbIY6ui0RycSr9qsWB0CSNLYgYnRPYwi8Xt2He9g$" target="_blank">
Outlook for Android</a><o:p></o:p></p>
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<p class="MsoNormal"><b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black">From:</span></b><span style="font-size:11.0pt;font-family:"Calibri",sans-serif;color:black"> Jason Zhu <<a href="mailto:jasonz13@uci.edu" target="_blank">jasonz13@uci.edu</a>><br>
<b>Sent:</b> Tuesday, July 18, 2023 8:57:55 PM<br>
<b>To:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Cc:</b> James S. Nowick <<a href="mailto:jsnowick@uci.edu" target="_blank">jsnowick@uci.edu</a>>; Henrickson, Amy <<a href="mailto:amy.henrickson@uleth.ca" target="_blank">amy.henrickson@uleth.ca</a>>;
<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a> <<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a>>;
<a href="mailto:maduni.ranasinghe@uleth.ca" target="_blank">maduni.ranasinghe@uleth.ca</a> <<a href="mailto:maduni.ranasinghe@uleth.ca" target="_blank">maduni.ranasinghe@uleth.ca</a>>; Bowler, Bruce <<a href="mailto:Bruce.Bowler@mso.umt.edu" target="_blank">Bruce.Bowler@mso.umt.edu</a>>;
Frederick, Ariel <<a href="mailto:ariel.frederick@umconnect.umt.edu" target="_blank">ariel.frederick@umconnect.umt.edu</a>><br>
<b>Subject:</b> Re: AUC Collaboration: Sizing Amyloid Beta derived Oligomers</span>
<o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal">Hi Borries and James, <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I tried running different TFA concentrations on the nanodrop and got very noisy UV-Vis spectra that I don't fully trust. I enabled the automatic baseline correction and automatic pathlength adjustment settings on the nanodrop which seems
to have helped with the negative absorbance values that don't make physical sense. I will meet with James tomorrow morning and will work out the details of getting accurate absorbance values for both TFA and F19Cha then.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><img border="0" width="562" height="495" style="width:5.8541in;height:5.1562in" id="_x0000_i1026" src="cid:image001.png@01D9BA2C.F6EF5E30" alt="image.png"><o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I plan on weighing out the peptides, labeling them, and shipping them by the end of the day Wednesday (tomorrow). I'll send an update once we figure out the ideal wavelength for the different concentrations we plan to run at each concentration
and when the peptide is on its way.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">For lyophilized powder, 4C should be adequate. F19Cha isn't prone to oxidizing from my experience working with it.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Best,<o:p></o:p></p>
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<p class="MsoNormal">Jason<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">On Tue, Jul 18, 2023 at 2:38 PM Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>> wrote:<o:p></o:p></p>
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<p class="MsoNormal">Hi Bruce and Ariel:<o:p></o:p></p>
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<p class="MsoNormal">We would like to have some samples shipped to U of Montana, and I would like to pick them up on Friday, if this is possible? The samples are lyophilized peptide samples and can probably be stored at 4C. I will be in Missoula on Friday
and Saturday, all day, our entire lab will actually be in Missoula for a vaccine development workshop with the CTM group. Thanks for helping again with the samples!!<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">James and Jason:<o:p></o:p></p>
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<p class="MsoNormal">Regarding the sample shipment, you can overnight them as a lyophilized powder to:
<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">University of Montana<o:p></o:p></p>
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<p class="MsoNormal">Dept. of Chemistry &Biochemistry<o:p></o:p></p>
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<p class="MsoNormal">c/o Bruce Bowler or Ariel Frederick<o:p></o:p></p>
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<p class="MsoNormal">23 Campus Drive<o:p></o:p></p>
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<p class="MsoNormal">Missoula, Montana 59812<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Please weigh them out and indicate on your shipping list with what volume they need to be suspended to get the desired concentrations. Our loading volume will be 110 ul, and we would like to know the wavelength to use where we would get
0.6 OD in a 3 mm pathlength centerpiece, with no more than 0.3 OD of background absorbance from the buffer (when blanked against water). We can use different wavelengths for each sample to optimize the absorbance for each concentration.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">The speed will be 60 krpm, so they should be measured in the An60Ti rotor, at 20C.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Please ship so they will be available for pickup by us on Friday this week. Please indicate the storage conditions - I assume for the lyophilized powder 4C is adequate?<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Thanks, -Borries<o:p></o:p></p>
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