<html><head><meta http-equiv="content-type" content="text/html; charset=utf-8"></head><body style="overflow-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">Wonderful! Thanks, Bruce!<div>James<br><div><br><blockquote type="cite"><div>On Jul 21, 2023, at 10:21 AM, Bowler, Bruce <Bruce.Bowler@mso.umt.edu> wrote:</div><br class="Apple-interchange-newline"><div><meta charset="UTF-8"><div class="WordSection1" style="page: WordSection1; caret-color: rgb(0, 0, 0); font-family: ArialMT; font-size: 16px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;"><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);">Hi Borries,<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);"><o:p> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);">The samples arrived about an hour ago and are in my -20 freezer.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);"><o:p> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);">I will mostly be around my lab until about 2:45 pm today.<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);"><o:p> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);">Cheers,<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);">Bruce<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);"><o:p> </o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><b><span style="font-size: 11pt; font-family: Calibri, sans-serif;">From:</span></b><span style="font-size: 11pt; font-family: Calibri, sans-serif;"><span class="Apple-converted-space"> </span>Borries Demeler <<a href="mailto:demeler@gmail.com" style="color: purple; text-decoration: underline;">demeler@gmail.com</a>><span class="Apple-converted-space"> </span><br><b>Sent:</b><span class="Apple-converted-space"> </span>Wednesday, July 19, 2023 9:26 PM<br><b>To:</b><span class="Apple-converted-space"> </span>Jason Zhu <<a href="mailto:jasonz13@uci.edu" style="color: purple; text-decoration: underline;">jasonz13@uci.edu</a>><br><b>Cc:</b><span class="Apple-converted-space"> </span>James S. Nowick <<a href="mailto:jsnowick@uci.edu" style="color: purple; text-decoration: underline;">jsnowick@uci.edu</a>>; Henrickson, Amy <<a href="mailto:amy.henrickson@uleth.ca" style="color: purple; text-decoration: underline;">amy.henrickson@uleth.ca</a>>;<span class="Apple-converted-space"> </span><a href="mailto:demelerlab@biophysics.uleth.ca" style="color: purple; text-decoration: underline;">demelerlab@biophysics.uleth.ca</a>;<span class="Apple-converted-space"> </span><a href="mailto:maduni.ranasinghe@uleth.ca" style="color: purple; text-decoration: underline;">maduni.ranasinghe@uleth.ca</a>; Bowler, Bruce <<a href="mailto:Bruce.Bowler@mso.umt.edu" style="color: purple; text-decoration: underline;">Bruce.Bowler@mso.umt.edu</a>>; Frederick, Ariel <<a href="mailto:ariel.frederick@umconnect.umt.edu" style="color: purple; text-decoration: underline;">ariel.frederick@umconnect.umt.edu</a>><br><b>Subject:</b><span class="Apple-converted-space"> </span>Re: AUC Collaboration: Sizing Amyloid Beta derived Oligomers<o:p></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Perfect! Thanks so much, and thanks to Bruce for handling our samples, I will check in with you during the morning session of our workshop. Amy, please remind me in case I forget.<o:p></o:p></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Thanks, -Borries<o:p></o:p></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">On Wed, Jul 19, 2023 at 9:24 PM Jason Zhu <<a href="mailto:jasonz13@uci.edu" style="color: purple; text-decoration: underline;">jasonz13@uci.edu</a>> wrote:<o:p></o:p></div></div><blockquote style="border-width: medium medium medium 1pt; border-style: none none none solid; border-color: currentcolor currentcolor currentcolor rgb(204, 204, 204); border-image: none; padding: 0in 0in 0in 6pt; margin-left: 4.8pt; margin-right: 0in;"><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Hi Bruce, Ariel, and Borries,<o:p></o:p></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">The peptide (F19Cha) should ship tomorrow (Thursday) morning and arrive before 10:30 am on Friday. I've attached the shipping label and instructions for how much water to add to make 1, 3, and 9 mM samples. <o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Best,<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Jason<o:p></o:p></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">On Wed, Jul 19, 2023 at 11:59 AM James S. Nowick <<a href="mailto:jsnowick@uci.edu" target="_blank" style="color: purple; text-decoration: underline;">jsnowick@uci.edu</a>> wrote:<o:p></o:p></div></div><blockquote style="border-width: medium medium medium 1pt; border-style: none none none solid; border-color: currentcolor currentcolor currentcolor rgb(204, 204, 204); border-image: none; padding: 0in 0in 0in 6pt; margin-left: 4.8pt; margin-right: 0in;"><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Hi All,<o:p></o:p></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">The bottom line is that the absorbance of trifluoroacetate should be negligible with respect to the peptide. The peptide absorbs so strongly at 214 nm that you will need to go to longer wavelengths. Alternatively, I think interference optics might be a good option.<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Best,<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">James<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><br><br><o:p></o:p></div><blockquote style="margin-top: 5pt; margin-bottom: 5pt;"><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">On Jul 18, 2023, at 7:57 PM, Jason Zhu <<a href="mailto:jasonz13@uci.edu" target="_blank" style="color: purple; text-decoration: underline;">jasonz13@uci.edu</a>> wrote:<o:p></o:p></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Hi Borries and James,<o:p></o:p></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">I tried running different TFA concentrations on the nanodrop and got very noisy UV-Vis spectra that I don't fully trust. I enabled the automatic baseline correction and automatic pathlength adjustment settings on the nanodrop which seems to have helped with the negative absorbance values that don't make physical sense. I will meet with James tomorrow morning and will work out the details of getting accurate absorbance values for both TFA and F19Cha then.<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><image.png><o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">I plan on weighing out the peptides, labeling them, and shipping them by the end of the day Wednesday (tomorrow). I'll send an update once we figure out the ideal wavelength for the different concentrations we plan to run at each concentration and when the peptide is on its way.<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">For lyophilized powder, 4C should be adequate. F19Cha isn't prone to oxidizing from my experience working with it.<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Best,<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Jason<o:p></o:p></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">On Tue, Jul 18, 2023 at 2:38 PM Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank" style="color: purple; text-decoration: underline;">demeler@gmail.com</a>> wrote:<o:p></o:p></div></div><blockquote style="border-width: medium medium medium 1pt; border-style: none none none solid; border-color: currentcolor currentcolor currentcolor rgb(204, 204, 204); border-image: none; padding: 0in 0in 0in 6pt; margin-left: 4.8pt; margin-right: 0in;"><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Hi Bruce and Ariel:<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">We would like to have some samples shipped to U of Montana, and I would like to pick them up on Friday, if this is possible? The samples are lyophilized peptide samples and can probably be stored at 4C. I will be in Missoula on Friday and Saturday, all day, our entire lab will actually be in Missoula for a vaccine development workshop with the CTM group. Thanks for helping again with the samples!!<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">James and Jason:<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Regarding the sample shipment, you can overnight them as a lyophilized powder to:<o:p></o:p></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">University of Montana<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Dept. of Chemistry &Biochemistry<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">c/o Bruce Bowler or Ariel Frederick<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">23 Campus Drive<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Missoula, Montana 59812<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Please weigh them out and indicate on your shipping list with what volume they need to be suspended to get the desired concentrations. Our loading volume will be 110 ul, and we would like to know the wavelength to use where we would get 0.6 OD in a 3 mm pathlength centerpiece, with no more than 0.3 OD of background absorbance from the buffer (when blanked against water). We can use different wavelengths for each sample to optimize the absorbance for each concentration.<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">The speed will be 60 krpm, so they should be measured in the An60Ti rotor, at 20C.<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Please ship so they will be available for pickup by us on Friday this week. Please indicate the storage conditions - I assume for the lyophilized powder 4C is adequate?<o:p></o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div></div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">Thanks, -Borries<o:p></o:p></div></div></div></div></blockquote></div></div></blockquote></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;"><o:p> </o:p></div><div><div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: "Times New Roman", serif;">James S. Nowick<br>Distinguished Professor<br>Department of Chemistry & Department of Pharmaceutical Sciences<br><br>Department of Chemistry<br>4126 Natural Sciences 1<br>University of California, Irvine<br>Irvine, CA 92697-2025<br><br>Phone: (949) 824-6091<br>e-mail:<span class="Apple-converted-space"> </span><a href="mailto:jsnowick@uci.edu" target="_blank" style="color: purple; text-decoration: underline;">jsnowick@uci.edu</a><br>Faculty Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/jsnowick/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!N1pni7MvQC2b2B8UkuEuyJfTSy3SNOX8O3u82A0rJS42kQP5CMvnPCF331ntQaU-5VV5CG3ejXgg-gw0D2sQwB0-qA$" target="_blank" style="color: purple; text-decoration: underline;">http://tinyurl.com/jsnowick/</a><br>Research Group Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/nowickgroup/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!N1pni7MvQC2b2B8UkuEuyJfTSy3SNOX8O3u82A0rJS42kQP5CMvnPCF331ntQaU-5VV5CG3ejXgg-gw0D2ux34kS7g$" target="_blank" style="color: purple; text-decoration: underline;">http://tinyurl.com/nowickgroup/</a><br>Pronouns: he/him/his</div></div></div></div></div></blockquote></div></blockquote></div></div></div></blockquote></div><br><div>
<meta charset="UTF-8"><div>James S. Nowick<br>Distinguished Professor<br>Department of Chemistry & Department of Pharmaceutical Sciences<br><br>Department of Chemistry<br>4126 Natural Sciences 1<br>University of California, Irvine<br>Irvine, CA 92697-2025<br><br>Phone: (949) 824-6091<br>e-mail: jsnowick@uci.edu<br>Faculty Web Page: http://tinyurl.com/jsnowick/<br>Research Group Web Page: http://tinyurl.com/nowickgroup/<br>Pronouns: he/him/his</div>
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