<div dir="ltr"><div>Hi Borries and Amy,</div><div><br></div><div>Here's the zoomed in spectra from the Nanodrop that you requested with the 10 mm absorbance in the 0-3 OD range. I also normalized the Y-axis to a 3 mm absorbance in that range in case it would be helpful to look at. I can look into the dynamic and linear absorbance range of the instrument to see how much we should trust these UV-Vis spectra.</div><div><br></div><div>Also, if the experiment works better with the 10 mm centerpieces for the interference optics, I can provide more material as needed. </div><img src="cid:ii_lkix2mul2" alt="image.png" width="465" height="319" style="margin-right: 0px;"><br><div><img src="cid:ii_lkiwtl3g1" alt="image.png" width="464" height="319" style="margin-right: 0px;"><br></div><div><br></div><div>Best,</div><div>Jason</div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, Jul 25, 2023 at 4:06 PM Henrickson, Amy <<a href="mailto:amy.henrickson@uleth.ca">amy.henrickson@uleth.ca</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div>
<div>
<div>
<div dir="ltr">
<div dir="ltr">We do have a cuvette that only needs 60uL of samples. It does have a little more noise than the ones we usually use but for getting in idea of OD it should work fine. </div>
<div dir="ltr"><br>
</div>
<div dir="ltr">-Amy</div>
</div>
</div>
<div id="m_-5621342544037036762ms-outlook-mobile-signature">
<div><br>
</div>
Get <a href="https://urldefense.com/v3/__https://aka.ms/o0ukef__;!!CzAuKJ42GuquVTTmVmPViYEvSg!I66z_m6fiIkwmzYpyzCJLFt0VGQ-2-OlyMNthYqnMISq08OOrEdooCYoBTQ6TF9AS40WZ6ErdAjMV40tiWAEXQ7xayb3$" target="_blank">Outlook for iOS</a></div>
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<hr style="display:inline-block;width:98%">
<div id="m_-5621342544037036762divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Sent:</b> Tuesday, July 25, 2023 4:59:24 PM<br>
<b>To:</b> Henrickson, Amy <<a href="mailto:amy.henrickson@uleth.ca" target="_blank">amy.henrickson@uleth.ca</a>><br>
<b>Cc:</b> James S. Nowick <<a href="mailto:jsnowick@uci.edu" target="_blank">jsnowick@uci.edu</a>>; Jason Zhu <<a href="mailto:jasonz13@uci.edu" target="_blank">jasonz13@uci.edu</a>>; <a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a> <<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a>>; Ranasinghe, Maduni <<a href="mailto:maduni.ranasinghe@uleth.ca" target="_blank">maduni.ranasinghe@uleth.ca</a>><br>
<b>Subject:</b> Re: AUC Collaboration: Sizing Amyloid Beta derived Oligomers</font>
<div> </div>
</div>
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<b>outside of the University of Lethbridge</b>. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to <a href="mailto:phishing@uleth.ca" target="_blank">phishing@uleth.ca</a>.</span></p>
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<div>
<div dir="ltr">Amy, keep in mind that some of the samples at ~110 ul for the 3 mm centerpieces may be too low volume for our 1 cm pathlength cuvettes, and I don't think we want to dilute until we have enough volume so we can still measure at high enough concentration.
As James suggested, interference could be an option, though I am not sure if the 3 mm centerpieces actually are in the correct focus plane for the interference optics, something we need to confirm first. They should be in the 2/3 plane, but are in the 0.5
plane. Maybe this discrepancy is not critical, and there is enough material so we can make this measurement. Either way, we'll figure it out, just something to think about.
<div><br>
</div>
<div>Jason, assuming the Y-axis is correct, can you please send us a plot where the Y-axis is zoomed to 0-3 OD region because anything over 3 OD in a 10 mm centerpiece would be too absorbing to be measured in a 3 mm centerpiece.<br>
<div><br>
</div>
<div>Thanks! -Borries<br>
<div><br>
</div>
<div><br>
</div>
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<div>
<div dir="ltr">On Tue, Jul 25, 2023 at 4:41 PM Henrickson, Amy <<a href="mailto:amy.henrickson@uleth.ca" target="_blank">amy.henrickson@uleth.ca</a>> wrote:<br>
</div>
<blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
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<div dir="ltr">
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Hello, </div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
<br>
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<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
I think that we will just collect spectra of the samples on our instrument before we measure them anyway. This will likely be easier for everyone. </div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Jason sent a document saying how much water to resuspend every sample in so we can do that and measure them to see what wavelength we should measure at. </div>
<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
<br>
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Regarding the dissolving of the sample, I know that it said the type of water was important. We have milli-q water we could use of water for injection. Which would be better?</div>
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<br>
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<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Thanks, </div>
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<br>
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<div style="font-family:Calibri,Arial,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)">
Amy HEnrickson </div>
<div id="m_-5621342544037036762x_m_8154544295709162903appendonsend"></div>
<hr style="display:inline-block;width:98%">
<div id="m_-5621342544037036762x_m_8154544295709162903divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> James S. Nowick <<a href="mailto:jsnowick@uci.edu" target="_blank">jsnowick@uci.edu</a>><br>
<b>Sent:</b> Tuesday, July 25, 2023 3:20 PM<br>
<b>To:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Cc:</b> Jason Zhu <<a href="mailto:jasonz13@uci.edu" target="_blank">jasonz13@uci.edu</a>>; Henrickson, Amy <<a href="mailto:amy.henrickson@uleth.ca" target="_blank">amy.henrickson@uleth.ca</a>>;
<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a> <<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a>>; Ranasinghe, Maduni <<a href="mailto:maduni.ranasinghe@uleth.ca" target="_blank">maduni.ranasinghe@uleth.ca</a>><br>
<b>Subject:</b> Re: AUC Collaboration: Sizing Amyloid Beta derived Oligomers</font>
<div> </div>
</div>
<div>
<div style="border:1pt solid rgb(100,100,100);padding:2pt;background:rgb(255,235,156)">
<p style="line-height:12pt;margin:5px"><b><span style="font-size:10pt;color:rgb(156,101,0)"></span></b><span style="font-size:10pt;color:black">Caution: This email was sent from someone
<b>outside of the University of Lethbridge</b>. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to
<a href="mailto:phishing@uleth.ca" target="_blank">phishing@uleth.ca</a>.</span></p>
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<br>
<div>Hi Borries,
<div><br>
</div>
<div>I’m pretty sure these a run on the NanoDrop spectrophotometer. The NanoDrop has a variable pathlength of 0.03-1.0 mm and typically normalizes the Y-axis to the equivalent of a 1.0 cm pathlength. As a result, it can give readings for concentrated solutions
of up to 550 absorbance units. .</div>
<div><br>
</div>
<div>Best,</div>
<div>James<br>
<div><br>
<blockquote type="cite">
<div>On Jul 25, 2023, at 1:55 PM, Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>> wrote:</div>
<br>
<div>
<div dir="ltr">I am wholly confused about the y axis scale and label on your plot. It says 10 mm absorbance (i.e., 1 cm cuvette?). You should get values in the 0.0-1.0 range, not in the hundreds of OD. Assuming all of these measurements are within the dynamic
range of the detector, we should be able to use any wavelength between 210-240 nm. We will adjust the wavelength so that any concentration will absorb within 0.3-0.7 OD in a 3 mm centerpiece. Good to know that water+TFA against water does not absorb at all,
thanks for measuring this. It would still help us to know what the actual scale on the y axis is. Can you send a corrected plot?
<div><br>
</div>
<div>Thanks! -Borries</div>
</div>
<br>
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<div dir="ltr">On Tue, Jul 25, 2023 at 1:36 PM Jason Zhu <<a href="mailto:jasonz13@uci.edu" target="_blank">jasonz13@uci.edu</a>> wrote:<br>
</div>
<blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div dir="ltr">
<div>I reran the UV-vis spectra of water + 0.1% TFA, 1 mM F19Cha, 3 mM F19Cha, and 9 mM F19Cha all blanked against water and it worked much better this time. The TFA absorbs negligibly above ~220 nm and absorbs weakly compared to that of F19Cha. To get a 0.6
absorbance in a 3 mm centerpiece for the 1, 3, and 9 mM samples with less than 0.3 background absorbance, I would recommend 268, 278, and 279 nm respectively. I'm open to adjusting the wavelengths as you see fit. I've attached the Excel for the spectra below.
I think the reason why I got negative absorbance last time was because the nanopure water that I was blanking with had some quality control issues. </div>
<div><br>
</div>
<div>Please feel free to reach out if there's anything you need from my end!</div>
<div><br>
</div>
<div>Best,</div>
<div>Jason</div>
<div><span id="m_-5621342544037036762x_m_8154544295709162903x_cid:ii_lkioov2b0"><image.png></span><br>
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<div><br>
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<div>Best,</div>
<div>Jason</div>
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<br>
<div>
<div dir="ltr">On Fri, Jul 21, 2023 at 10:47 AM James S. Nowick <<a href="mailto:jsnowick@uci.edu" target="_blank">jsnowick@uci.edu</a>> wrote:<br>
</div>
<blockquote style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
<div>Wonderful! Thanks, Bruce!
<div>James<br>
<div><br>
<blockquote type="cite">
<div>On Jul 21, 2023, at 10:21 AM, Bowler, Bruce <<a href="mailto:Bruce.Bowler@mso.umt.edu" target="_blank">Bruce.Bowler@mso.umt.edu</a>> wrote:</div>
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<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)">Hi Borries,<u></u><u></u></span></div>
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<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)"><u></u> <u></u></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman",serif">
<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)">The samples arrived about an hour ago and are in my -20 freezer.<u></u><u></u></span></div>
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<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)"><u></u> <u></u></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman",serif">
<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)">I will mostly be around my lab until about 2:45 pm today.<u></u><u></u></span></div>
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<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)"><u></u> <u></u></span></div>
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<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)">Cheers,<u></u><u></u></span></div>
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<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)">Bruce<u></u><u></u></span></div>
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<span style="font-size:11pt;font-family:Calibri,sans-serif;color:rgb(31,73,125)"><u></u> <u></u></span></div>
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<b><span style="font-size:11pt;font-family:Calibri,sans-serif">From:</span></b><span style="font-size:11pt;font-family:Calibri,sans-serif"><span> </span>Borries Demeler <<a href="mailto:demeler@gmail.com" style="color:purple;text-decoration:underline" target="_blank">demeler@gmail.com</a>><span> </span><br>
<b>Sent:</b><span> </span>Wednesday, July 19, 2023 9:26 PM<br>
<b>To:</b><span> </span>Jason Zhu <<a href="mailto:jasonz13@uci.edu" style="color:purple;text-decoration:underline" target="_blank">jasonz13@uci.edu</a>><br>
<b>Cc:</b><span> </span>James S. Nowick <<a href="mailto:jsnowick@uci.edu" style="color:purple;text-decoration:underline" target="_blank">jsnowick@uci.edu</a>>; Henrickson, Amy <<a href="mailto:amy.henrickson@uleth.ca" style="color:purple;text-decoration:underline" target="_blank">amy.henrickson@uleth.ca</a>>;<span> </span><a href="mailto:demelerlab@biophysics.uleth.ca" style="color:purple;text-decoration:underline" target="_blank">demelerlab@biophysics.uleth.ca</a>;<span> </span><a href="mailto:maduni.ranasinghe@uleth.ca" style="color:purple;text-decoration:underline" target="_blank">maduni.ranasinghe@uleth.ca</a>;
Bowler, Bruce <<a href="mailto:Bruce.Bowler@mso.umt.edu" style="color:purple;text-decoration:underline" target="_blank">Bruce.Bowler@mso.umt.edu</a>>; Frederick, Ariel <<a href="mailto:ariel.frederick@umconnect.umt.edu" style="color:purple;text-decoration:underline" target="_blank">ariel.frederick@umconnect.umt.edu</a>><br>
<b>Subject:</b><span> </span>Re: AUC Collaboration: Sizing Amyloid Beta derived Oligomers<u></u><u></u></span></div>
<div style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman",serif">
<u></u> <u></u></div>
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Perfect! Thanks so much, and thanks to Bruce for handling our samples, I will check in with you during the morning session of our workshop. Amy, please remind me in case I forget.<u></u><u></u></div>
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Thanks, -Borries<u></u><u></u></div>
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<u></u> <u></u></div>
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On Wed, Jul 19, 2023 at 9:24 PM Jason Zhu <<a href="mailto:jasonz13@uci.edu" style="color:purple;text-decoration:underline" target="_blank">jasonz13@uci.edu</a>> wrote:<u></u><u></u></div>
</div>
<blockquote style="border-width:medium medium medium 1pt;border-style:none none none solid;border-color:currentcolor currentcolor currentcolor rgb(204,204,204);padding:0in 0in 0in 6pt;margin-left:4.8pt;margin-right:0in">
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Hi Bruce, Ariel, and Borries,<u></u><u></u></div>
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<u></u> <u></u></div>
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The peptide (F19Cha) should ship tomorrow (Thursday) morning and arrive before 10:30 am on Friday. I've attached the shipping label and instructions for how much water to add to make 1, 3, and 9 mM samples. <u></u><u></u></div>
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<u></u> <u></u></div>
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Best,<u></u><u></u></div>
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Jason<u></u><u></u></div>
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<u></u> <u></u></div>
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On Wed, Jul 19, 2023 at 11:59 AM James S. Nowick <<a href="mailto:jsnowick@uci.edu" style="color:purple;text-decoration:underline" target="_blank">jsnowick@uci.edu</a>> wrote:<u></u><u></u></div>
</div>
<blockquote style="border-width:medium medium medium 1pt;border-style:none none none solid;border-color:currentcolor currentcolor currentcolor rgb(204,204,204);padding:0in 0in 0in 6pt;margin-left:4.8pt;margin-right:0in">
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Hi All,<u></u><u></u></div>
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<u></u> <u></u></div>
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The bottom line is that the absorbance of trifluoroacetate should be negligible with respect to the peptide. The peptide absorbs so strongly at 214 nm that you will need to go to longer wavelengths. Alternatively, I think interference optics might be a good
option.<u></u><u></u></div>
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<u></u> <u></u></div>
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Best,<u></u><u></u></div>
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James<u></u><u></u></div>
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<u></u> <u></u></div>
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<br>
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<u></u><u></u></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman",serif">
On Jul 18, 2023, at 7:57 PM, Jason Zhu <<a href="mailto:jasonz13@uci.edu" style="color:purple;text-decoration:underline" target="_blank">jasonz13@uci.edu</a>> wrote:<u></u><u></u></div>
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<u></u> <u></u></div>
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Hi Borries and James,<u></u><u></u></div>
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<u></u> <u></u></div>
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<div style="margin:0in 0in 0.0001pt;font-size:12pt;font-family:"Times New Roman",serif">
I tried running different TFA concentrations on the nanodrop and got very noisy UV-Vis spectra that I don't fully trust. I enabled the automatic baseline correction and automatic pathlength adjustment settings on the nanodrop which seems to have helped with
the negative absorbance values that don't make physical sense. I will meet with James tomorrow morning and will work out the details of getting accurate absorbance values for both TFA and F19Cha then.<u></u><u></u></div>
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<u></u> <u></u></div>
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<image.png><u></u><u></u></div>
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<u></u> <u></u></div>
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I plan on weighing out the peptides, labeling them, and shipping them by the end of the day Wednesday (tomorrow). I'll send an update once we figure out the ideal wavelength for the different concentrations we plan to run at each concentration and when the
peptide is on its way.<u></u><u></u></div>
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<u></u> <u></u></div>
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For lyophilized powder, 4C should be adequate. F19Cha isn't prone to oxidizing from my experience working with it.<u></u><u></u></div>
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Best,<u></u><u></u></div>
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Jason<u></u><u></u></div>
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On Tue, Jul 18, 2023 at 2:38 PM Borries Demeler <<a href="mailto:demeler@gmail.com" style="color:purple;text-decoration:underline" target="_blank">demeler@gmail.com</a>> wrote:<u></u><u></u></div>
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Hi Bruce and Ariel:<u></u><u></u></div>
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We would like to have some samples shipped to U of Montana, and I would like to pick them up on Friday, if this is possible? The samples are lyophilized peptide samples and can probably be stored at 4C. I will be in Missoula on Friday and Saturday, all day,
our entire lab will actually be in Missoula for a vaccine development workshop with the CTM group. Thanks for helping again with the samples!!<u></u><u></u></div>
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James and Jason:<u></u><u></u></div>
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Regarding the sample shipment, you can overnight them as a lyophilized powder to:<u></u><u></u></div>
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University of Montana<u></u><u></u></div>
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Dept. of Chemistry &Biochemistry<u></u><u></u></div>
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c/o Bruce Bowler or Ariel Frederick<u></u><u></u></div>
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23 Campus Drive<u></u><u></u></div>
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Missoula, Montana 59812<u></u><u></u></div>
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Please weigh them out and indicate on your shipping list with what volume they need to be suspended to get the desired concentrations. Our loading volume will be 110 ul, and we would like to know the wavelength to use where we would get 0.6 OD in a 3 mm pathlength
centerpiece, with no more than 0.3 OD of background absorbance from the buffer (when blanked against water). We can use different wavelengths for each sample to optimize the absorbance for each concentration.<u></u><u></u></div>
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The speed will be 60 krpm, so they should be measured in the An60Ti rotor, at 20C.<u></u><u></u></div>
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Please ship so they will be available for pickup by us on Friday this week. Please indicate the storage conditions - I assume for the lyophilized powder 4C is adequate?<u></u><u></u></div>
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Thanks, -Borries<u></u><u></u></div>
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James S. Nowick<br>
Distinguished Professor<br>
Department of Chemistry & Department of Pharmaceutical Sciences<br>
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Department of Chemistry<br>
4126 Natural Sciences 1<br>
University of California, Irvine<br>
Irvine, CA 92697-2025<br>
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Phone: (949) 824-6091<br>
e-mail:<span> </span><a href="mailto:jsnowick@uci.edu" style="color:purple;text-decoration:underline" target="_blank">jsnowick@uci.edu</a><br>
Faculty Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/jsnowick/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!N1pni7MvQC2b2B8UkuEuyJfTSy3SNOX8O3u82A0rJS42kQP5CMvnPCF331ntQaU-5VV5CG3ejXgg-gw0D2sQwB0-qA$" style="color:purple;text-decoration:underline" target="_blank">http://tinyurl.com/jsnowick/</a><br>
Research Group Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/nowickgroup/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!N1pni7MvQC2b2B8UkuEuyJfTSy3SNOX8O3u82A0rJS42kQP5CMvnPCF331ntQaU-5VV5CG3ejXgg-gw0D2ux34kS7g$" style="color:purple;text-decoration:underline" target="_blank">http://tinyurl.com/nowickgroup/</a><br>
Pronouns: he/him/his</div>
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<div>James S. Nowick<br>
Distinguished Professor<br>
Department of Chemistry & Department of Pharmaceutical Sciences<br>
<br>
Department of Chemistry<br>
4126 Natural Sciences 1<br>
University of California, Irvine<br>
Irvine, CA 92697-2025<br>
<br>
Phone: (949) 824-6091<br>
e-mail: <a href="mailto:jsnowick@uci.edu" target="_blank">jsnowick@uci.edu</a><br>
Faculty Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/jsnowick/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!Pa-b8ObzdUvFuCSfCNJqhfvF9uZw9D0Dot6lNb2qzJbQwrgtD-kp20F44c3pcW9Gn9XoPbUSbTRH_n4$" target="_blank">http://tinyurl.com/jsnowick/</a><br>
Research Group Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/nowickgroup/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!Pa-b8ObzdUvFuCSfCNJqhfvF9uZw9D0Dot6lNb2qzJbQwrgtD-kp20F44c3pcW9Gn9XoPbUS6KQo-ck$" target="_blank">http://tinyurl.com/nowickgroup/</a><br>
Pronouns: he/him/his</div>
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<div>James S. Nowick<br>
Distinguished Professor<br>
Department of Chemistry & Department of Pharmaceutical Sciences<br>
<br>
Department of Chemistry<br>
4126 Natural Sciences 1<br>
University of California, Irvine<br>
Irvine, CA 92697-2025<br>
<br>
Phone: (949) 824-6091<br>
e-mail: <a href="mailto:jsnowick@uci.edu" target="_blank">jsnowick@uci.edu</a><br>
Faculty Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/jsnowick/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!I66z_m6fiIkwmzYpyzCJLFt0VGQ-2-OlyMNthYqnMISq08OOrEdooCYoBTQ6TF9AS40WZ6ErdAjMV40tiWAEXTG8kOht$" target="_blank">http://tinyurl.com/jsnowick/</a><br>
Research Group Web Page: <a href="https://urldefense.com/v3/__http://tinyurl.com/nowickgroup/__;!!CzAuKJ42GuquVTTmVmPViYEvSg!I66z_m6fiIkwmzYpyzCJLFt0VGQ-2-OlyMNthYqnMISq08OOrEdooCYoBTQ6TF9AS40WZ6ErdAjMV40tiWAEXW-STnfk$" target="_blank">http://tinyurl.com/nowickgroup/</a><br>
Pronouns: he/him/his</div>
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