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Hi Vince and Andres,</div>
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Considering that the samples arrived at room temperature (the exact length of time is unknown), and that an ice pack leaked (the samples appear to have been sealed properly). We are just looking to confirm that you feel it is worth continuing to process this
batch of samples, taking into account your insight into the temperature-stability of the proteins at ambient temperature. <span style="display: inline !important; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted2"></span></div>
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If so, as per the updated experimental design in the LIMS, we will start out running both<span style="display: inline !important; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><span> </span>CanA and Hyper2</span> at a high and low concentration
in the EDTA-containing buffer at 20 degrees Celsius. To accomplish this, I need to know w<span style="display: inline !important; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted3">hat buffer each of the protein stocks are in
and whether or not they contain EDTA. </span> </div>
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<span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;"><br>
</span></div>
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<span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">If this experiment is successful, we will repeat in the presence of calcium ions (10mM for CanA, and 1mM for Hyper2). For this we need to know at what temperature and for how
long the samples should be incubated with the CaCl2 solution. Finally, if this experiment is successful, we will look at repeating with increased calcium ion concentrations, as well as running at a higher temperature such as 37 degrees Celsius.</span><br>
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</span></div>
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<span style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 12pt;">I'll report back on the results from the first run, which will take place this week.</span></div>
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<font face="Calibri, Helvetica, sans-serif"><span style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif; color: rgb(0, 0, 0);">Cheers,</span></font></div>
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<span style="font-size: 12pt; font-family: Calibri, Helvetica, sans-serif; color: rgb(0, 0, 0);">Reece</span></div>
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<span data-ogsc="" style="margin: 0px;"><span data-ogsc="" style="font-family: verdana, sans-serif; margin: 0px;"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc="" style=""><span data-ogsc="" style="margin: 0px;" class="ContentPasted0"><span class="ContentPasted0" style=""><span style="font-family: Calibri, Helvetica, sans-serif; font-size: 9pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">--</span></span></span></font></span></span></span></div>
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<span data-ogsc="" style="margin: 0px;"><span data-ogsc="" style="font-family: verdana, sans-serif; margin: 0px;"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc="" style=""><span data-ogsc="" style="margin: 0px;" class="ContentPasted0"><span class="ContentPasted0" style=""><span style="font-family: Calibri, Helvetica, sans-serif; font-size: 9pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"></span></span></span></font></span></span></span></div>
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<span data-ogsc="" style="margin:0px"><span data-ogsc="" style="font-family:verdana, sans-serif;margin:0px"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc=""><span data-ogsc="" style="margin:0px"><span style="margin:0px"><span style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">Reece
Martin,</span><span style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1"> </span></span><span data-ogsc="" data-ogsb="white" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">B.Sc</span></span></font></span></span></span>
<div data-ogsc="" style="margin: 0px; background-color: white;"><span data-ogsc="" style="font-family:verdana, sans-serif;margin:0px"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc=""><span data-ogsc="" style="margin:0px"><span data-ogsc="" data-ogsb="white" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">M.Sc.
Candidate</span></span></font></span></span></div>
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<span data-ogsc="" style="font-family:verdana, sans-serif;margin:0px"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc=""><span data-ogsc="" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">Northwest
Biophysics Consortium</span></font></span></span></div>
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<span data-ogsc="" style="font-family:verdana, sans-serif;margin:0px"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc=""><span data-ogsc="" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">Alberta
RNA Research and Training Institute (ARRTI)</span><span data-ogsc="" style="margin:0px"><br class="ContentPasted1">
</span></font></span></span></div>
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<span data-ogsc="" style="font-family:verdana, sans-serif;margin:0px"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc=""><span data-ogsc="" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">Lab
of Dr. Borries Demeler</span></font></span></span></div>
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of Chemistry and Biochemistry</span><span data-ogsc="" style="margin:0px"><br class="ContentPasted1">
</span><span data-ogsc="" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">University of Lethbridge</span><br class="ContentPasted1">
</font></span></span></div>
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<div style=""><span dir="ltr" data-ogsc="rgb(50, 49, 48)" data-ogsb="white" style="font-size: 15px; font-family: Calibri, Arial, Helvetica, sans-serif; margin: 0px; color: rgb(50, 49, 48); background-color: white;"><span data-ogsc="" style="font-family:verdana, sans-serif;margin:0px"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc=""><span data-ogsc="" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">Email:</span><span data-ogsc="" style="margin:0px"><span style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1"> reece.martin</span></span><span data-ogsc="" style="font-size: 9pt; font-family: Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted1">@uleth.ca</span></font></span></span></span></div>
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403-498-5882</span></font></span></span></span><span dir="ltr" data-ogsc="rgb(50, 49, 48)" data-ogsb="white" style="font-family: Calibri, Arial, Helvetica, sans-serif; font-size: 15px; margin: 0px; color: rgb(50, 49, 48); background-color: white;"><span data-ogsc="" style="font-family:verdana, sans-serif;margin:0px"><span data-ogsc="black" style="margin: 0px; color: black;"><font size="1" data-ogsc=""><span data-ogsc="" style="margin: 0px; font-family: Calibri, Helvetica, sans-serif; font-size: 9pt; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);" class="ContentPasted0"></span></font></span></span></span><br>
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