<div dir="ltr">Perfect, I reviewed the project request and I have most of the information I need to design the experiment. I was wondering if you could add a representative TEM image by uploading it in your LIMS account under "Supporting Files" (button in the left bar). You can associate it with your project request. Also, if you know the expected range of particle sizes in nanometers that would help as well to predict the most suitable rotor speed, and would save some experimentation time. If you happen to have an absorbance spectrum down to 220 nm or so of a very dilute sample, you can upload that as well.<div><br></div><div>As soon as I have approval from Nick to proceed, Sophia in our lab will send you shipping instructions. Please include at least 15 ml of buffer for these samples for dilutions.</div><div><br></div><div>Thanks! -Borries<br><div><br></div><div><br></div></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Tue, Sep 19, 2023 at 8:27 AM Samantha R. Tracy <<a href="mailto:Samantha@clene.com">Samantha@clene.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div class="msg-6845471628153378996">
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Hi Borries,</div>
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We have just filled out the project details to the best of our ability. If you have any follow up questions let me know.</div>
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Thanks,</div>
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<b style="font-style:inherit;font-variant-ligatures:inherit;font-variant-caps:inherit;font-family:Calibri,sans-serif;font-size:14.6667px;color:rgb(32,31,30)"><span style="font-size:10pt;font-family:Helvetica,sans-serif;color:rgb(21,91,154)">Samantha
Tracy</span></b><br>
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<span style="font-size:9pt;color:rgb(51,51,51)">Research Engineer</span></div>
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<div id="m_-6845471628153378996divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Sent:</b> Monday, September 18, 2023 5:51 PM<br>
<b>To:</b> Raj, Rekha M <<a href="mailto:RRAJ@beckman.com" target="_blank">RRAJ@beckman.com</a>>; Harrington, Nick (Nicholas) J <<a href="mailto:NJHARRINGTON@beckman.com" target="_blank">NJHARRINGTON@beckman.com</a>>; Samantha R. Tracy <<a href="mailto:Samantha@clene.com" target="_blank">Samantha@clene.com</a>>; Mikhail Merzliakov <<a href="mailto:Mikhail@clene.com" target="_blank">Mikhail@clene.com</a>><br>
<b>Cc:</b> Aysha Demeler <<a href="mailto:aysha.demeler@uleth.ca" target="_blank">aysha.demeler@uleth.ca</a>>; Elie Chdid <<a href="mailto:elie@aucsolutions.com" target="_blank">elie@aucsolutions.com</a>>; Luc Roberts <<a href="mailto:luc@aucsolutions.com" target="_blank">luc@aucsolutions.com</a>><br>
<b>Subject:</b> EXTERNAL: Gold nanoparticles</font>
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<div dir="ltr">Hi everyone,
<div>as discussed today, in order to demonstrate the feasibility of the AUC method, and to contrast the results obtained from DLS, we plan to compare three samples of gold nanoparticles by sedimentation velocity. The goal is to demonstrate that AUC can pick
up particle size distributions with much higher resolution than DLS and also can differentiate between different densities resulting from different metal core-hydration shell ratios.</div>
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<div>These questions have been previously evaluated by AUC in the following publications, two of which Aysha has contributed significantly already:</div>
<div><a href="https://pubmed.ncbi.nlm.nih.gov/25010012/" target="_blank"><br>
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<div><a href="https://pubmed.ncbi.nlm.nih.gov/25010012/" target="_blank">Demeler B, Nguyen TL, Gorbet GE, Schirf V, Brookes EH, Mulvaney P, El-Ballouli AO, Pan J, Bakr OM, Demeler AK, Hernandez Uribe BI, Bhattarai N, Whetten RL. Characterization of size, anisotropy, and density
heterogeneity of nanoparticles by sedimentation velocity. Anal Chem. 2014 Aug 5;86(15):7688-95. doi: 10.1021/ac501722r. Epub 2014 Jul 18. PMID: 25010012; PMCID: PMC4144751.</a><br>
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<div>and:</div>
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<div><a href="https://pubmed.ncbi.nlm.nih.gov/24739173/" target="_blank">Gorbet G, Devlin T, Hernandez Uribe BI, Demeler AK, Lindsey ZL, Ganji S, Breton S, Weise-Cross L, Lafer EM, Brookes EH, Demeler B. A parametrically constrained optimization method for fitting sedimentation
velocity experiments. Biophys J. 2014 Apr 15;106(8):1741-50. doi: 10.1016/j.bpj.2014.02.022. PMID: 24739173; PMCID: PMC4008825.</a></div>
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<div>Core-shell studies by analytical ultracentrifugation have been done by us as well:</div>
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<div><span style="color:rgb(33,33,33);font-size:16px"><a href="https://pubmed.ncbi.nlm.nih.gov/27934989/" target="_blank">Walter J, Gorbet G, Akdas T, Segets D, Demeler B, Peukert W. 2D analysis of polydisperse core-shell nanoparticles using analytical ultracentrifugation.
Analyst. 2016 Dec 19;142(1):206-217. doi: 10.1039/c6an02236g. PMID: 27934989; PMCID: PMC5167643.</a></span><br>
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<div>To proceed, we ask that the scientist in charge of this project creates a LIMS account in our LIMS server instance on this website:</div>
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<div><a href="https://uslims.uleth.ca/uslims3_AUCCANADA/newaccount.php" target="_blank">https://uslims.uleth.ca/uslims3_AUCCANADA/newaccount.php<br>
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<div>and create a project request ("Projects" button on the left bar) to get the ball rolling. Aysha will provide you with the shipping instructions and Luc or Elie will prepare a cost estimate for Nick to get a PO issued. We discussed obtaining ~2 ml of 1
OD at peak absorbance wavelength of three different samples to demonstrate that different answers can be obtained for each sample, and we may consider diluting the sample with the storage buffer (please provide 15 ml). Further details will be provided in the
project request.</div>
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<div>Please let me know if you have any questions.</div>
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<div>Regards, -Borries</div>
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