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Hello all,</div>
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I am a student in Bo's lab and just wanted to provide some shipping information. To avoid any issues with customs, samples could be sent to the University of Montana and Bo can pick them up by this Thursday. B<span style="color: rgb(0, 0, 0);">ruce Bowler can
receive them for us, his contact information is below:</span></div>
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<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">University of Montana</span><span data-ogsc="rgb(31, 73, 125)" style="font-size: 11pt; font-family: Calibri, sans-serif; margin: 0px; color: rgb(31, 73, 125);" class="ContentPasted0"><u data-ogsc="" class="ContentPasted0"></u><u data-ogsc="" class="ContentPasted0"></u></span></p>
<p class="x_MsoNormal" style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; font-size: 15px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">
<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">32 Campus Dr</span><span data-ogsc="rgb(31, 73, 125)" style="font-size: 11pt; font-family: Calibri, sans-serif; margin: 0px; color: rgb(31, 73, 125);" class="ContentPasted0"><u data-ogsc="" class="ContentPasted0"></u><u data-ogsc="" class="ContentPasted0"></u></span></p>
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<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">Missoula, MT 59812</span><span data-ogsc="rgb(31, 73, 125)" style="font-size: 11pt; font-family: Calibri, sans-serif; margin: 0px; color: rgb(31, 73, 125);" class="ContentPasted0"><u data-ogsc="" class="ContentPasted0"></u><u data-ogsc="" class="ContentPasted0"></u></span></p>
<p class="x_MsoNormal" style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; font-size: 15px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">
<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">Attn: Bruce Bowler, Chemistry and Biochemistry
(Chem 310)</span><span data-ogsc="rgb(31, 73, 125)" style="font-size: 11pt; font-family: Calibri, sans-serif; margin: 0px; color: rgb(31, 73, 125);" class="ContentPasted0"><u data-ogsc="" class="ContentPasted0"></u><u data-ogsc="" class="ContentPasted0"></u></span></p>
<p class="x_MsoNormal" style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; font-size: 15px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">
<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">Phone: 406 243-6114</span></p>
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<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">I've attached a commercial invoice with the receiver
information. Again, the experimental design was updated to outline the plan. Please ensure that 15 mL of buffer and the two 1.5 mL aliquots at 1 OD 525 nm are provided. </span></p>
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<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0"><br>
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<p class="x_MsoNormal" style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; font-size: 15px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">
<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">Let me know if there are any questions!</span></p>
<p class="x_MsoNormal" style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; font-size: 15px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">
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<p class="x_MsoNormal" style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; font-size: 15px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">
<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">Thanks,</span></p>
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</span></p>
<p class="x_MsoNormal" style="font-family: "Segoe UI", "Segoe UI Web (West European)", "Segoe UI", -apple-system, BlinkMacSystemFont, Roboto, "Helvetica Neue", sans-serif; font-size: 15px; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">
<span data-ogsc="rgb(31, 73, 125)" style="font-size: 12pt; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; margin: 0px; color: rgb(0, 0, 0);" class="ContentPasted0">Sophia</span></p>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Demelerlab <demelerlab-bounces@biophysics.uleth.ca> on behalf of Harrington, Nick (Nicholas) J <NJHARRINGTON@beckman.com><br>
<b>Sent:</b> 19 September 2023 10:33<br>
<b>To:</b> Borries Demeler <demeler@gmail.com>; Samantha R. Tracy <Samantha@clene.com>; demelerlab@biophysics.uleth.ca <demelerlab@biophysics.uleth.ca><br>
<b>Subject:</b> Re: [Demelerlab] EXTERNAL: Gold nanoparticles</font>
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<p class="x_MsoNormal">Thanks Borries for leading the call yesterday and apologies to the group for my unexpected absence due to dental work and delays at their office. Ugh.</p>
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<p class="x_MsoNormal">The proposed experimentation, number of samples, and expected output is clear and fits with our expectations. Please have a quotation put together and sent to my attention for review. Once I have that, I will approve and we can move
forward with sample shipping and subsequent analysis. </p>
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<p class="x_MsoNormal">Thanks!</p>
<p class="x_MsoNormal">Nick<span style="font-size:9.0pt; font-family:"Arial",sans-serif"><br>
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Confidential - Company Proprietary<br>
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<p class="x_MsoNormal"><b>From:</b> Borries Demeler <demeler@gmail.com> <br>
<b>Sent:</b> Tuesday, September 19, 2023 11:58 AM<br>
<b>To:</b> Samantha R. Tracy <Samantha@clene.com>; demelerlab@biophysics.uleth.ca; Harrington, Nick (Nicholas) J <NJHARRINGTON@beckman.com><br>
<b>Subject:</b> Re: EXTERNAL: Gold nanoparticles</p>
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<p class="x_MsoNormal" style=""><span style="font-size:1.0pt; color:white">Perfect, I reviewed the project request and I have most of the information I need to design the experiment. I was wondering if you could add a representative TEM image by uploading it
in your LIMS account under "Supporting Files" </span></p>
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<p class="x_MsoNormal">Perfect, I reviewed the project request and I have most of the information I need to design the experiment. I was wondering if you could add a representative TEM image by uploading it in your LIMS account under "Supporting Files" (button
in the left bar). You can associate it with your project request. Also, if you know the expected range of particle sizes in nanometers that would help as well to predict the most suitable rotor speed, and would save some experimentation time. If you happen
to have an absorbance spectrum down to 220 nm or so of a very dilute sample, you can upload that as well.</p>
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<p class="x_MsoNormal">As soon as I have approval from Nick to proceed, Sophia in our lab will send you shipping instructions. Please include at least 15 ml of buffer for these samples for dilutions.</p>
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<p class="x_MsoNormal">Thanks! -Borries</p>
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<p class="x_MsoNormal">On Tue, Sep 19, 2023 at 8:27 AM Samantha R. Tracy <<a href="mailto:Samantha@clene.com">Samantha@clene.com</a>> wrote:</p>
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<p class="x_MsoNormal"><span style="font-size:12.0pt; color:black">Hi Borries,</span></p>
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<p class="x_MsoNormal"><span style="font-size:12.0pt; color:black">We have just filled out the project details to the best of our ability. If you have any follow up questions let me know.</span></p>
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<p class="x_MsoNormal"><span style="font-size:12.0pt; color:black"> </span></p>
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<p class="x_MsoNormal"><span style="font-size:12.0pt; color:black">Thanks,</span></p>
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<p class="x_MsoNormal"><b><span style="font-size:10.0pt; font-family:"Helvetica",sans-serif; color:#155B9A">Samantha Tracy</span></b><span style="font-size:12.0pt; color:black"></span></p>
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<p class="x_MsoNormal"><span style="font-size:9.0pt; color:#333333">Research Engineer</span><span style="font-size:12.0pt; color:black"></span></p>
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<p class="x_MsoNormal"><span style="font-size:9.0pt; color:#333333">Clene Nanomedicine</span><span style="font-size:12.0pt; color:black"></span></p>
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<p class="x_MsoNormal"><b><span style="color:black">From:</span></b><span style="color:black"> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Sent:</b> Monday, September 18, 2023 5:51 PM<br>
<b>To:</b> Raj, Rekha M <<a href="mailto:RRAJ@beckman.com" target="_blank">RRAJ@beckman.com</a>>; Harrington, Nick (Nicholas) J <<a href="mailto:NJHARRINGTON@beckman.com" target="_blank">NJHARRINGTON@beckman.com</a>>; Samantha R. Tracy <<a href="mailto:Samantha@clene.com" target="_blank">Samantha@clene.com</a>>;
Mikhail Merzliakov <<a href="mailto:Mikhail@clene.com" target="_blank">Mikhail@clene.com</a>><br>
<b>Cc:</b> Aysha Demeler <<a href="mailto:aysha.demeler@uleth.ca" target="_blank">aysha.demeler@uleth.ca</a>>; Elie Chdid <<a href="mailto:elie@aucsolutions.com" target="_blank">elie@aucsolutions.com</a>>; Luc Roberts <<a href="mailto:luc@aucsolutions.com" target="_blank">luc@aucsolutions.com</a>><br>
<b>Subject:</b> EXTERNAL: Gold nanoparticles</span> </p>
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<p class="x_MsoNormal">Hi everyone, </p>
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<p class="x_MsoNormal">as discussed today, in order to demonstrate the feasibility of the AUC method, and to contrast the results obtained from DLS, we plan to compare three samples of gold nanoparticles by sedimentation velocity. The goal is to demonstrate
that AUC can pick up particle size distributions with much higher resolution than DLS and also can differentiate between different densities resulting from different metal core-hydration shell ratios.</p>
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<p class="x_MsoNormal"> </p>
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<p class="x_MsoNormal">These questions have been previously evaluated by AUC in the following publications, two of which Aysha has contributed significantly already:</p>
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<p class="x_MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=" target="_blank"><br>
</a></p>
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<p class="x_MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=" target="_blank">Demeler
B, Nguyen TL, Gorbet GE, Schirf V, Brookes EH, Mulvaney P, El-Ballouli AO, Pan J, Bakr OM, Demeler AK, Hernandez Uribe BI, Bhattarai N, Whetten RL. Characterization of size, anisotropy, and density heterogeneity of nanoparticles by sedimentation velocity.
Anal Chem. 2014 Aug 5;86(15):7688-95. doi: 10.1021/ac501722r. Epub 2014 Jul 18. PMID: 25010012; PMCID: PMC4144751.</a></p>
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<p class="x_MsoNormal">and:</p>
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<p class="x_MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_24739173_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=je35GYrI-3jZcUoJuixhZqJO35FJ0Y86rViw-Nz7HxY&e=" target="_blank">Gorbet
G, Devlin T, Hernandez Uribe BI, Demeler AK, Lindsey ZL, Ganji S, Breton S, Weise-Cross L, Lafer EM, Brookes EH, Demeler B. A parametrically constrained optimization method for fitting sedimentation velocity experiments. Biophys J. 2014 Apr 15;106(8):1741-50.
doi: 10.1016/j.bpj.2014.02.022. PMID: 24739173; PMCID: PMC4008825.</a></p>
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<p class="x_MsoNormal">Core-shell studies by analytical ultracentrifugation have been done by us as well:</p>
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<p class="x_MsoNormal"><span style="font-size:12.0pt; color:#212121"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_27934989_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=fWF0XIeLJLYPXBfDcwQZ581eJyQWsdQPfGmYGvXj8iw&e=" target="_blank">Walter
J, Gorbet G, Akdas T, Segets D, Demeler B, Peukert W. 2D analysis of polydisperse core-shell nanoparticles using analytical ultracentrifugation. Analyst. 2016 Dec 19;142(1):206-217. doi: 10.1039/c6an02236g. PMID: 27934989; PMCID: PMC5167643.</a></span></p>
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<p class="x_MsoNormal">To proceed, we ask that the scientist in charge of this project creates a LIMS account in our LIMS server instance on this website:</p>
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<p class="x_MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=" target="_blank">https://uslims.uleth.ca/uslims3_AUCCANADA/newaccount.php<br>
</a></p>
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<p class="x_MsoNormal"><a href="https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=" target="_blank"><br>
</a></p>
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<p class="x_MsoNormal">and create a project request ("Projects" button on the left bar) to get the ball rolling. Aysha will provide you with the shipping instructions and Luc or Elie will prepare a cost estimate for Nick to get a PO issued. We discussed obtaining
~2 ml of 1 OD at peak absorbance wavelength of three different samples to demonstrate that different answers can be obtained for each sample, and we may consider diluting the sample with the storage buffer (please provide 15 ml). Further details will be provided
in the project request.</p>
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<p class="x_MsoNormal">Please let me know if you have any questions.</p>
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<p class="x_MsoNormal">Regards, -Borries</p>
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