<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
</head>
<body style="overflow-wrap: break-word; -webkit-nbsp-mode: space; line-break: after-white-space;">
Hei Sophia,
<div><br>
</div>
<div>Glad to hear from you!</div>
<div>I just submitted a new project in LIMS giving details on the proposed experiments and background for the project.</div>
<div><br>
</div>
<div>Regarding meeting on zoom, next week would be better for me (I am traveling for US thanksgiving weekend), Monday I am available all day (except from 1.30-3 pm pacific time), Tuesday I am available after 11.30 pacific time, and Thursday I am available all
day. Let me know what works best for you.</div>
<div><br>
</div>
<div>Look forward to meeting you on zoom.</div>
<div><br>
</div>
<div>Best,</div>
<div>Josefine </div>
<div><br>
</div>
<div>
<div><br>
<blockquote type="cite">
<div>20. nov. 2023 kl. 08:36 skrev Bird, Sophia <sophia.bird@uleth.ca>:</div>
<br class="Apple-interchange-newline">
<div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
Hello Josefine,</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
<br>
</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
I am a lab tech working in the Demeler lab and will be helping organize instrument time and experimental planning for your project. Let's schedule a meeting to discuss the experimental design so we can get started. Please communicate your availability this
week or next week and submit a new project in your LIMS account before the meeting so we can familiarize ourselves with it.</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
<br>
</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
Let me know if you have any questions!</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
<br>
</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
Best,</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
<br>
</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
Sophia</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
<br>
</div>
<div class="elementToProof" style="font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt;">
<br>
</div>
<div id="appendonsend" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;">
</div>
<hr tabindex="-1" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; display: inline-block; width: 847.6875px;">
<span style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none; float: none; display: inline !important;"></span>
<div id="divRplyFwdMsg" dir="ltr" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;">
<font face="Calibri, sans-serif" style="font-size: 11pt;"><b>From:</b><span class="Apple-converted-space"> </span>Demelerlab <<a href="mailto:demelerlab-bounces@biophysics.uleth.ca">demelerlab-bounces@biophysics.uleth.ca</a>> on behalf of Josefine Eilsoe Nielsen
<<a href="mailto:josefine@stanford.edu">josefine@stanford.edu</a>><br>
<b>Sent:</b><span class="Apple-converted-space"> </span>05 November 2023 14:35<br>
<b>To:</b><span class="Apple-converted-space"> </span>Borries Demeler <<a href="mailto:demeler@gmail.com">demeler@gmail.com</a>><br>
<b>Cc:</b><span class="Apple-converted-space"> </span><a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a><span class="Apple-converted-space"> </span><<a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a>>;
Annelise E. Barron <<a href="mailto:aebarron@stanford.edu">aebarron@stanford.edu</a>><br>
<b>Subject:</b><span class="Apple-converted-space"> </span>Re: [Demelerlab] Update from the Barron lab</font>
<div> </div>
</div>
<div dir="auto" style="caret-color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; word-spacing: 0px; -webkit-text-stroke-width: 0px; text-decoration: none;">
<div style="border: 1pt solid rgb(100, 100, 100); padding: 2pt; background: rgb(255, 235, 156);">
<p class="x_MsoNormal" style="margin: 5px; line-height: 12pt;"><b><span style="font-size: 10pt; color: rgb(156, 101, 0);"></span></b><span style="font-size: 10pt;">Caution: This email was sent from someone<span class="Apple-converted-space"> </span><b>outside
of the University of Lethbridge</b>. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to<span class="Apple-converted-space"> </span><a href="mailto:phishing@uleth.ca">phishing@uleth.ca</a>.</span></p>
</div>
<br>
<div>
<div dir="ltr"></div>
<div dir="ltr">So sorry to hear about your car, I am glad you were able to return to Leithbridge safe! </div>
<div dir="ltr">Samples should be ok in kitchen freezer, no worries. </div>
<div dir="ltr"><br>
</div>
<div dir="ltr">Josefine</div>
<div dir="ltr"><br>
<blockquote type="cite">On 5 Nov 2023, at 13:19, Borries Demeler <demeler@gmail.com> wrote:<br>
<br>
</blockquote>
</div>
<blockquote type="cite">
<div dir="ltr">
<div dir="ltr">Hi Josefine,
<div>my car developed engine issues on the way down to Missoula, so returned to Lethbridge and will have to get my car fixed first. I hope I can get it fixed reasonably fast. Is the sample ok in a kitchen freezer?</div>
<div>-Borries</div>
</div>
<br>
<div class="x_gmail_quote">
<div dir="ltr" class="x_gmail_attr">On Thu, Nov 2, 2023 at 5:54 PM Josefine Eilsoe Nielsen <<a href="mailto:josefine@stanford.edu">josefine@stanford.edu</a>> wrote:<br>
</div>
<blockquote class="x_gmail_quote" style="margin: 0px 0px 0px 0.8ex; border-left-width: 1px; border-left-style: solid; border-left-color: rgb(204, 204, 204); padding-left: 1ex;">
<div>Hi,
<div><br>
</div>
<div>That sounds like a good plan (sorry to hear about the instrument issues though). The peptides in powder form should be stored at at -20C for longer periods (we usually keep them at -80C, but Anaspec reports -20 as sufficient), but can be shipped at room
temperature. Should we still ship them to Missoula?</div>
<div><br>
</div>
<div>Yes, I did check my LIMS account and could log in without issues, but I was having issues connecting using VNC, but I will try to download Ultrascan and connect my database as suggested. Will let you know if I run into any issues. </div>
<div><br>
</div>
<div>Best,</div>
<div>Josefine<br>
<div><br>
<blockquote type="cite">
<div>31. okt. 2023 kl. 05:10 skrev Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>>:</div>
<br>
<div>
<div dir="ltr">Hi Josefine,
<div>I am glad to hear you guys figured out a way to prepare stable and colloidal systems of abeta and LL37, <br>
<div>I would be happy to help you with AUC to measure the LL37/abeta complex. Does ammonium hydroxide absorb in the low UV? If not, we can stick to low concentrations at low UV wavelengths and measure at low concentration. With a detailed dissolving protocol
sending the powder should work fine. It may be a while before we can get to it since one of our machines is down right now. I would recommend sending the material to Missoula, since it is stable (it should be fine at 4C, correct?). Then I could pick it up
the next time I am down here (driving back to Lethbridge today).</div>
<div><br>
</div>
<div>Regarding the UltraScan access, you can either install UltraScan on your system (<a href="https://www.ultrascan3.aucsolutions.com/download.php" target="_blank">https://www.ultrascan3.aucsolutions.com/download.php</a>) and then connect it to your account
in the CCH database ("Database login info" once you are logged in), or via VNC directly on our server. I could help you with either approach over Zoom if needed. It is possible we will have to recreate your account because inactive accounts get removed after
a while. I checked, and your LIMS account with all of your data is still active and ready to go.</div>
</div>
<div><br>
</div>
<div>Let me know how you want to proceed.</div>
<div><br>
</div>
<div>Regards, -Borries</div>
</div>
</div>
</blockquote>
</div>
</div>
</div>
</blockquote>
</div>
</div>
</blockquote>
</div>
</div>
</div>
</blockquote>
</div>
<br>
</div>
</body>
</html>