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<span style="font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif;">Hi Josefine,</span></div>
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<span style="font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif;">Thank you for your project submission, that is very helpful! Thursday at 10 am MT/ 9 am PT works for us here, I will send a calendar invite for the scheduled
discussion.</span></div>
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<span style="font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif;">Best,</span></div>
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<span style="font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif;">Sophia</span></div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Josefine Eilsoe Nielsen <josefine@stanford.edu><br>
<b>Sent:</b> 22 November 2023 13:21<br>
<b>To:</b> Bird, Sophia <sophia.bird@uleth.ca><br>
<b>Cc:</b> Borries Demeler <demeler@gmail.com>; demelerlab@biophysics.uleth.ca <demelerlab@biophysics.uleth.ca>; Annelise E. Barron <aebarron@stanford.edu><br>
<b>Subject:</b> Re: Update from the Barron lab</font>
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<b>outside of the University of Lethbridge</b>. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to phishing@uleth.ca.</span></p>
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<div>Hei Sophia,
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<div>Glad to hear from you!</div>
<div>I just submitted a new project in LIMS giving details on the proposed experiments and background for the project.</div>
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<div>Regarding meeting on zoom, next week would be better for me (I am traveling for US thanksgiving weekend), Monday I am available all day (except from 1.30-3 pm pacific time), Tuesday I am available after 11.30 pacific time, and Thursday I am available all
day. Let me know what works best for you.</div>
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<div>Look forward to meeting you on zoom.</div>
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<div>Best,</div>
<div>Josefine </div>
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<div>20. nov. 2023 kl. 08:36 skrev Bird, Sophia <sophia.bird@uleth.ca>:</div>
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Hello Josefine,</div>
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I am a lab tech working in the Demeler lab and will be helping organize instrument time and experimental planning for your project. Let's schedule a meeting to discuss the experimental design so we can get started. Please communicate your availability this
week or next week and submit a new project in your LIMS account before the meeting so we can familiarize ourselves with it.</div>
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Let me know if you have any questions!</div>
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Best,</div>
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Sophia</div>
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<font face="Calibri, sans-serif" style="font-size:11pt"><b>From:</b><span class="x_Apple-converted-space"> </span>Demelerlab <<a href="mailto:demelerlab-bounces@biophysics.uleth.ca">demelerlab-bounces@biophysics.uleth.ca</a>> on behalf of Josefine Eilsoe Nielsen
<<a href="mailto:josefine@stanford.edu">josefine@stanford.edu</a>><br>
<b>Sent:</b><span class="x_Apple-converted-space"> </span>05 November 2023 14:35<br>
<b>To:</b><span class="x_Apple-converted-space"> </span>Borries Demeler <<a href="mailto:demeler@gmail.com">demeler@gmail.com</a>><br>
<b>Cc:</b><span class="x_Apple-converted-space"> </span><a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a><span class="x_Apple-converted-space"> </span><<a href="mailto:demelerlab@biophysics.uleth.ca">demelerlab@biophysics.uleth.ca</a>>;
Annelise E. Barron <<a href="mailto:aebarron@stanford.edu">aebarron@stanford.edu</a>><br>
<b>Subject:</b><span class="x_Apple-converted-space"> </span>Re: [Demelerlab] Update from the Barron lab</font>
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<div dir="ltr">So sorry to hear about your car, I am glad you were able to return to Leithbridge safe! </div>
<div dir="ltr">Samples should be ok in kitchen freezer, no worries. </div>
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<div dir="ltr">Josefine</div>
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<blockquote type="cite">On 5 Nov 2023, at 13:19, Borries Demeler <demeler@gmail.com> wrote:<br>
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<div dir="ltr">Hi Josefine,
<div>my car developed engine issues on the way down to Missoula, so returned to Lethbridge and will have to get my car fixed first. I hope I can get it fixed reasonably fast. Is the sample ok in a kitchen freezer?</div>
<div>-Borries</div>
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<div dir="ltr" class="x_x_gmail_attr">On Thu, Nov 2, 2023 at 5:54 PM Josefine Eilsoe Nielsen <<a href="mailto:josefine@stanford.edu">josefine@stanford.edu</a>> wrote:<br>
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<div>Hi,
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<div>That sounds like a good plan (sorry to hear about the instrument issues though). The peptides in powder form should be stored at at -20C for longer periods (we usually keep them at -80C, but Anaspec reports -20 as sufficient), but can be shipped at room
temperature. Should we still ship them to Missoula?</div>
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<div>Yes, I did check my LIMS account and could log in without issues, but I was having issues connecting using VNC, but I will try to download Ultrascan and connect my database as suggested. Will let you know if I run into any issues. </div>
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<div>Best,</div>
<div>Josefine<br>
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<div>31. okt. 2023 kl. 05:10 skrev Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>>:</div>
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<div dir="ltr">Hi Josefine,
<div>I am glad to hear you guys figured out a way to prepare stable and colloidal systems of abeta and LL37, <br>
<div>I would be happy to help you with AUC to measure the LL37/abeta complex. Does ammonium hydroxide absorb in the low UV? If not, we can stick to low concentrations at low UV wavelengths and measure at low concentration. With a detailed dissolving protocol
sending the powder should work fine. It may be a while before we can get to it since one of our machines is down right now. I would recommend sending the material to Missoula, since it is stable (it should be fine at 4C, correct?). Then I could pick it up
the next time I am down here (driving back to Lethbridge today).</div>
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<div>Regarding the UltraScan access, you can either install UltraScan on your system (<a href="https://www.ultrascan3.aucsolutions.com/download.php" target="_blank">https://www.ultrascan3.aucsolutions.com/download.php</a>) and then connect it to your account
in the CCH database ("Database login info" once you are logged in), or via VNC directly on our server. I could help you with either approach over Zoom if needed. It is possible we will have to recreate your account because inactive accounts get removed after
a while. I checked, and your LIMS account with all of your data is still active and ready to go.</div>
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<div>Let me know how you want to proceed.</div>
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<div>Regards, -Borries</div>
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