<div dir="ltr"><br>FYI...also please share with the Patel lab.<div>Thanks, -Borries<br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">---------- Forwarded message ---------<br>From: <strong class="gmail_sendername" dir="auto">Nehal Thakor</strong> <span dir="auto"><<a href="mailto:nthakor2022@gmail.com">nthakor2022@gmail.com</a>></span><br>Date: Mon, Nov 27, 2023 at 11:30 AM<br>Subject: Re: Preparative Ultracentrifuge Imbalance - 24/11/23<br>To: Borries Demeler <<a href="mailto:demeler@gmail.com">demeler@gmail.com</a>><br>Cc: Martin, Reece <<a href="mailto:reece.martin@uleth.ca">reece.martin@uleth.ca</a>>, Patel, Trushar <<a href="mailto:trushar.patel@uleth.ca">trushar.patel@uleth.ca</a>>, Hayes, Paul <<a href="mailto:p.hayes@uleth.ca">p.hayes@uleth.ca</a>>, Thakor, Nehalkumar <<a href="mailto:nthakor@uleth.ca">nthakor@uleth.ca</a>>, <<a href="mailto:maurice.needham@uleth.ca">maurice.needham@uleth.ca</a>>, Lakshmi Narasimha, Pavan <<a href="mailto:p.lakshminarasimha@uleth.ca">p.lakshminarasimha@uleth.ca</a>>, Hegde, Veda <<a href="mailto:veda.hegde@uleth.ca">veda.hegde@uleth.ca</a>>, Patel, Jinay <<a href="mailto:jinay.patel@uleth.ca">jinay.patel@uleth.ca</a>><br></div><br><br><div dir="ltr"><div>We will make gradients and will use the weighing machine in the ultracentrifuge area to balance the tubes so that my students do have to walk to the centrifuge from my research area. <br></div><div>Please use my official e-mail (<a href="mailto:nthakor@uleth.ca" target="_blank">nthakor@uleth.ca</a>) for faster communication.</div><div>Thanks,</div><div>Nehal<br></div></div><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Fri, Nov 24, 2023 at 4:12 PM Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex"><div dir="auto"><div>Fyi... We need some additional training of the Thakor lab members, these instruments should not be that much out of balance. -Borries <br><br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Fri, Nov 24, 2023, 16:00 Martin, Reece <<a href="mailto:reece.martin@uleth.ca" target="_blank">reece.martin@uleth.ca</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0px 0px 0px 0.8ex;border-left:1px solid rgb(204,204,204);padding-left:1ex">
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<span style="font-family:Calibri,Helvetica,sans-serif">Hi Bo and Trushar,</span></div>
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<div><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"> Another run on the
</span><span style="letter-spacing:normal;font-family:Calibri,sans-serif;font-size:16px;font-weight:400;color:rgb(0,0,0)">Beckman Coulter Optima
</span><span style="letter-spacing:normal;font-family:Calibri,sans-serif;font-size:16px;font-weight:400;color:black">XPN-100</span><span style="letter-spacing:normal;font-family:Calibri,sans-serif;font-size:16px;font-weight:400;color:rgb(0,0,0)"> Preparative
Ultracentrifuge</span><span style="font-family:Calibri,Helvetica,sans-serif;font-size:12pt;color:rgb(0,0,0)"> was stopped due to an A700 imbalance error. This error suggests the tubes were out of balance by at least 5 mL or 10% of the total volume
(whichever was greater). The user was from the Thakor lab, and the imbalance was properly reported in the logbook (see attached image). Feel free to forward this information to whoever needs it.</span></div>
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<span style="font-family:Calibri,Helvetica,sans-serif">Cheers,</span></div>
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<span style="font-family:Calibri,Helvetica,sans-serif">Reece</span></div>
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</blockquote></div><br clear="all"><br><span class="gmail_signature_prefix">-- </span><br><div dir="ltr" class="gmail_signature"><div dir="ltr">Dr. Nehal Thakor, M.Sc., Ph.D. (he/him/his)<br><br>Associate Professor<br><br>Department of Chemistry and Biochemistry<br><br>The University of Lethbridge<br><br><a href="https://www.ulethbridge.ca/artsci/biochemistry/thakor-group" target="_blank">https://www.ulethbridge.ca/artsci/biochemistry/thakor-group</a><br><br> </div></div>
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