<html>
<head>
<meta http-equiv="Content-Type" content="text/html; charset=utf-8">
<style type="text/css" style="display:none;"> P {margin-top:0;margin-bottom:0;} </style>
</head>
<body dir="ltr">
<div class="elementToProof" style="font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
Hi Bo and Sophia,</div>
<div class="elementToProof" style="font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
<br>
</div>
<div class="elementToProof" style="font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 12pt; color: rgb(0, 0, 0);">
I discussed briefly with Walid about the results. <a href="mailto:sophia.bird@uleth.ca" id="OWAAM387211" class="tWKOu mention ms-bgc-nlr ms-fcl-b">
@Bird, Sophia</a> can you please send me the final report of the study? so I can make a presentation to the group to get other's opinion.</div>
<div class="elementToProof"><span style="letter-spacing: normal; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 16px; font-weight: 400; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">We might
want to try this study again with new labeled Spike protein and newly-made liposomes. I will let you know after that meeting.</span></div>
<div class="elementToProof"><span style="letter-spacing: normal; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 16px; font-weight: 400; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);"><br>
</span></div>
<div class="elementToProof"><span style="letter-spacing: normal; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 16px; font-weight: 400; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">Best,</span></div>
<div class="elementToProof"><span style="letter-spacing: normal; font-family: Aptos, Aptos_EmbeddedFont, Aptos_MSFontService, Calibri, Helvetica, sans-serif; font-size: 16px; font-weight: 400; color: rgb(0, 0, 0); background-color: rgb(255, 255, 255);">Bao</span></div>
<div id="appendonsend"></div>
<hr style="display:inline-block;width:98%" tabindex="-1">
<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Borries Demeler <demeler@gmail.com><br>
<b>Sent:</b> Tuesday, November 21, 2023 11:32 AM<br>
<b>To:</b> Le, Bao <bao.le@mso.umt.edu><br>
<b>Cc:</b> demelerlab@biophysics.uleth.ca <demelerlab@biophysics.uleth.ca>; Le, Minh <Minh.Le@mso.umt.edu><br>
<b>Subject:</b> Re: Spike Protein analysis</font>
<div> </div>
</div>
<div>
<div dir="ltr">Hi Bao,
<div>the limitation will be the signal we have from the labeled spike protein. We can deal with the baseline offset from the free label, and the remaining signal, while not a lot, should be sufficient to answer the composition questions when we mix it with
your lipids. Sophia is planning to run these experiments at the end of the week, so we should have further results early next week.</div>
<div><br>
</div>
<div>Best, -Borries</div>
</div>
<br>
<div class="x_gmail_quote">
<div dir="ltr" class="x_gmail_attr">On Tue, Nov 21, 2023 at 10:06 AM Le, Bao <<a href="mailto:bao.le@mso.umt.edu">bao.le@mso.umt.edu</a>> wrote:<br>
</div>
<blockquote class="x_gmail_quote" style="margin:0px 0px 0px 0.8ex; border-left:1px solid rgb(204,204,204); padding-left:1ex">
<div class="x_msg4154796150057947035">
<div dir="ltr">
<div style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0)">
Thank you, Borries, for the update!</div>
<div style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0)">
I hope we can have some meaningful data for a better understanding of the vaccine mechanism and also for publication
<span id="x_m_4154796150057947035🙂">🙂</span>.</div>
<div style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0)">
<br>
</div>
<div style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0)">
Best,</div>
<div><span style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0)">Bao</span></div>
<div id="x_m_4154796150057947035appendonsend"></div>
<hr style="display:inline-block; width:98%">
<div id="x_m_4154796150057947035divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" color="#000000" style="font-size:11pt"><b>From:</b> Borries Demeler <<a href="mailto:demeler@gmail.com" target="_blank">demeler@gmail.com</a>><br>
<b>Sent:</b> Monday, November 20, 2023 2:48 PM<br>
<b>To:</b> <a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a> <<a href="mailto:demelerlab@biophysics.uleth.ca" target="_blank">demelerlab@biophysics.uleth.ca</a>>; Le, Bao <<a href="mailto:bao.le@mso.umt.edu" target="_blank">bao.le@mso.umt.edu</a>>;
Le, Minh <<a href="mailto:Minh.Le@mso.umt.edu" target="_blank">Minh.Le@mso.umt.edu</a>><br>
<b>Subject:</b> Spike Protein analysis</font>
<div> </div>
</div>
<div>
<div dir="ltr">Hi Bao,
<div>Sophia measured your fluorescently labeled spike protein in our fluorescence instrument, and the signal was sufficient. However, it looks like most of the signal is coming from free fluorophore - which is OK, since we can eliminate it through data editing,
but the total signal from your protein is not as clean as it could be if the labeling had been more efficient. Just so you know... here is a picture:</div>
<div><br>
</div>
<div><img alt="image.png" width="644" height="516" style="margin-right:0px" data-outlook-trace="F:1|T:1" src="cid:ii_18bf325adb3cb971f161"><br>
</div>
<div>Sophia is planning to mix the remaining sample with the various lipids to see if there are any measurable interactions.</div>
<div><br>
</div>
<div>Best wishes, -Borries</div>
<div>Regards, -Borries</div>
</div>
</div>
</div>
</div>
</blockquote>
</div>
</div>
</body>
</html>