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<div dir="ltr">Dear Dr. Demeler,</div>
<div dir="ltr">I would suggest storing<span id="ms-outlook-ios-cursor"></span> the protein at -80 degree C for long term. Usually, I have seen this protein is stable, whenever I have worked with it. If it is possible, I would suggest keeping it on dry ice while
traveling to Canada. Because I know it doesn't precipitate at 4C but because we want to check the oligomerization state of this protein. I have no idea what will happen when we freeze thaw from -80 to 4, and then back to -80. </div>
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<div dir="ltr">Thank you!</div>
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<div dir="ltr">With best regards,</div>
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<div dir="ltr">Jyoti</div>
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<div id="divRplyFwdMsg" dir="ltr"><font face="Calibri, sans-serif" style="font-size:11pt" color="#000000"><b>From:</b> Borries Demeler <demeler@gmail.com><br>
<b>Sent:</b> Thursday, December 28, 2023 2:17:27 PM<br>
<b>To:</b> Baranwal, Jyoti <baranwal@uthscsa.edu><br>
<b>Cc:</b> Gupta, Yogesh K <GuptaY@uthscsa.edu>; demelerlab@biophysics.uleth.ca <demelerlab@biophysics.uleth.ca><br>
<b>Subject:</b> Re: Fw: FedEx shipment</font>
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<div>Great, thank you Jyoti! That should be more than sufficient to do all of the experiments. When I get back to Lethbridge we will schedule a zoom call with the rest of the lab so everyone is on the same page, and then we can discuss further details and confirm
our experimental designs. One more question: is the sample stable at 4C, or must it be stored at -80 or -20C? Can it be at 4C (on ice) while driving back to Canada - about 6 hours?<br>
Thanks, -Borries <br>
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<div dir="ltr" class="x_gmail_attr">On Thu, Dec 28, 2023, 12:59 Baranwal, Jyoti <<a href="mailto:baranwal@uthscsa.edu">baranwal@uthscsa.edu</a>> wrote:<br>
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Dear Dr. Demeler,</div>
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I have packed the sample and have sent it by overnight FedEx. Most probably it will reach you by tomorrow. <span>It contains 13 vials of 100ul, 13 mg/ml of protein (hope that is sufficient to try all different concentrations and buffers) and 10ml of 8 different
buffers listed below.</span></div>
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<ol style="font-size:15px; list-style:decimal; color:rgb(36,36,36); background-color:rgb(255,255,255)">
<li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"1. "; color:black">
<span style="margin:0px">200 mM LiSO4_25 mM Sodium acetate pH 4.5</span></li><li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"2. "; color:black">
<span style="margin:0px">15mM Tris-HCl_50mM KCl pH7.5</span></li><li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"3. "; color:black">
<span style="margin:0px">1x PBS</span></li><li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"4. "; color:black">
<span style="margin:0px">15mM Sodium acetate_100 mM NaCl pH 4.5</span></li><li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"5. "; color:black">
<span style="margin:0px">25mM MES_100mM NaCl pH 5.5</span></li><li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"6. "; color:black">
<span style="margin:0px">25mM MES_100mM NaCl pH 6.5</span></li><li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"7. "; color:black">
<span style="margin:0px">15mM Tris-HCl_100mM NaCl pH 7.5</span></li><li style="font-size:12pt; font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; list-style-type:"8. "; color:black">
<span style="margin:0px">15mM Tris-HCl_100mM NaCl pH 8.5</span></li></ol>
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<div style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0)">
<span>I have attached the FedEx tracking label with this email for your reference.</span></div>
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My sincere thanks to you!</div>
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<div style="font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif; font-size:12pt; color:rgb(0,0,0)">
<span>Jyoti Baranwal</span></div>
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<span style="font-size:9pt; color:rgb(12,100,192); background-color:rgb(255,255,255)"><b>Postdoctoral Researcher</b></span></div>
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<span style="font-size:9pt; color:rgb(12,100,192); background-color:rgb(255,255,255)"><b>Gupta Lab </b></span></div>
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<span style="font-size:9pt; color:rgb(12,100,192); background-color:rgb(255,255,255)"><b>Greehey Children's Cancer Research Institute (GCCRI)</b></span></div>
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<span style="font-size:9pt; color:rgb(12,100,192); background-color:rgb(255,255,255)"><b>University of Texas Health Science Center at San Antonio</b></span></div>
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<span style="font-size:9pt; color:rgb(12,100,192); background-color:rgb(255,255,255)"><b>Email:
<a href="mailto:baranwal@uthscsa.edu" target="_blank" rel="noreferrer">baranwal@uthscsa.edu</a></b></span></div>
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