<div dir="auto"><div>Great, thank you Jyoti! That should be more than sufficient to do all of the experiments. When I get back to Lethbridge we will schedule a zoom call with the rest of the lab so everyone is on the same page, and then we can discuss further details and confirm our experimental designs. One more question: is the sample stable at 4C, or must it be stored at -80 or -20C? Can it be at 4C (on ice) while driving back to Canada - about 6 hours?<br>Thanks, -Borries <br><div class="gmail_quote"><div dir="ltr" class="gmail_attr">On Thu, Dec 28, 2023, 12:59 Baranwal, Jyoti <<a href="mailto:baranwal@uthscsa.edu">baranwal@uthscsa.edu</a>> wrote:<br></div><blockquote class="gmail_quote" style="margin:0 0 0 .8ex;border-left:1px #ccc solid;padding-left:1ex">




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Dear Dr. Demeler,</div>
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I have packed the sample and have sent it by overnight FedEx. Most probably it will reach you by tomorrow. <span>It contains 13 vials of 100ul, 13 mg/ml of protein (hope that is sufficient to try all different concentrations and buffers)  and 10ml of 8 different
 buffers listed below.</span></div>
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<ol style="font-family:"Segoe UI","Segoe UI Web (West European)","Segoe UI",-apple-system,"system-ui",Roboto,"Helvetica Neue",sans-serif;font-size:15px;list-style:decimal;color:rgb(36,36,36);background-color:rgb(255,255,255)">
<li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"1. ";color:black">
<span style="margin:0px">200 mM LiSO4_25 mM Sodium acetate pH 4.5</span></li><li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"2. ";color:black">
<span style="margin:0px">15mM Tris-HCl_50mM KCl pH7.5</span></li><li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"3. ";color:black">
<span style="margin:0px">1x PBS</span></li><li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"4. ";color:black">
<span style="margin:0px">15mM Sodium acetate_100 mM NaCl pH 4.5</span></li><li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"5. ";color:black">
<span style="margin:0px">25mM MES_100mM NaCl pH 5.5</span></li><li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"6. ";color:black">
<span style="margin:0px">25mM MES_100mM NaCl pH 6.5</span></li><li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"7. ";color:black">
<span style="margin:0px">15mM Tris-HCl_100mM NaCl pH 7.5</span></li><li style="font-size:12pt;font-family:Aptos,Aptos_EmbeddedFont,Aptos_MSFontService,Calibri,Helvetica,sans-serif;list-style-type:"8. ";color:black">
<span style="margin:0px">15mM Tris-HCl_100mM NaCl pH 8.5</span></li></ol>
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<span>I have attached the FedEx tracking label with this email for your reference.</span></div>
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My sincere thanks to you!</div>
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<span>Jyoti Baranwal</span></div>
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<span style="font-size:9pt;color:rgb(12,100,192);background-color:rgb(255,255,255)"><b>Postdoctoral Researcher</b></span></div>
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<span style="font-size:9pt;color:rgb(12,100,192);background-color:rgb(255,255,255)"><b>Gupta Lab </b></span></div>
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<span style="font-size:9pt;color:rgb(12,100,192);background-color:rgb(255,255,255)"><b>Greehey Children's Cancer Research Institute (GCCRI)</b></span></div>
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<span style="font-size:9pt;color:rgb(12,100,192);background-color:rgb(255,255,255)"><b>University of Texas Health Science Center at San Antonio</b></span></div>
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<span style="font-size:9pt;color:rgb(12,100,192);background-color:rgb(255,255,255)"><b>Email: <a href="mailto:baranwal@uthscsa.edu" target="_blank" rel="noreferrer">baranwal@uthscsa.edu</a></b></span></div>
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