[Demelerlab] Spike Protein analysis

[Borries Demeler]

Sounds good, Bao, Sophia, can you please Cc me on the report you will send out?
Thanks, -Borries


On Tue, Dec 05, 2023 at 09:02:37PM +0000, Le, Bao wrote:
> Hi Bo and Sophia,
> 
> I discussed briefly with Walid about the results. @Bird, Sophia<mailto:sophia.bird at uleth.ca> can you please send me the final report of the study? so I can make a presentation to the group to get other's opinion.
> We might want to try this study again with new labeled Spike protein and newly-made liposomes. I will let you know after that meeting.
> 
> Best,
> Bao
> ________________________________
> From: Borries Demeler <demeler at gmail.com>
> Sent: Tuesday, November 21, 2023 11:32 AM
> To: Le, Bao <bao.le at mso.umt.edu>
> Cc: demelerlab at biophysics.uleth.ca <demelerlab at biophysics.uleth.ca>; Le, Minh <Minh.Le at mso.umt.edu>
> Subject: Re: Spike Protein analysis
> 
> Hi Bao,
> the limitation will be the signal we have from the labeled spike protein. We can deal with the baseline offset from the free label, and the remaining signal, while not a lot, should be sufficient to answer the composition questions when we mix it with your lipids. Sophia is planning to run these experiments at the end of the week, so we should have further results early next week.
> 
> Best, -Borries
> 
> On Tue, Nov 21, 2023 at 10:06 AM Le, Bao <bao.le at mso.umt.edu<mailto:bao.le at mso.umt.edu>> wrote:
> Thank you, Borries, for the update!
> I hope we can have some meaningful data for a better understanding of the vaccine mechanism and also for publication 🙂.
> 
> Best,
> Bao
> ________________________________
> From: Borries Demeler <demeler at gmail.com<mailto:demeler at gmail.com>>
> Sent: Monday, November 20, 2023 2:48 PM
> To: demelerlab at biophysics.uleth.ca<mailto:demelerlab at biophysics.uleth.ca> <demelerlab at biophysics.uleth.ca<mailto:demelerlab at biophysics.uleth.ca>>; Le, Bao <bao.le at mso.umt.edu<mailto:bao.le at mso.umt.edu>>; Le, Minh <Minh.Le at mso.umt.edu<mailto:Minh.Le at mso.umt.edu>>
> Subject: Spike Protein analysis
> 
> Hi Bao,
> Sophia measured your fluorescently labeled spike protein in our fluorescence instrument, and the signal was sufficient. However, it looks like most of the signal is coming from free fluorophore - which is OK, since we can eliminate it through data editing, but the total signal from your protein is not as clean as it could be if the labeling had been more efficient. Just so you know... here is a picture:
> 
> [image.png]
> Sophia is planning to mix the remaining sample with the various lipids to see if there are any measurable interactions.
> 
> Best wishes, -Borries
> Regards, -Borries