[Demelerlab] AUC Collaboration: Sizing Amyloid Beta derived Oligomers

[Borries Demeler]

Hi Bruce and Ariel:
We would like to have some samples shipped to U of Montana, and I would
like to pick them up on Friday, if this is possible? The samples are
lyophilized peptide samples and can probably be stored at 4C. I will be in
Missoula on Friday  and Saturday, all day, our entire lab will actually be
in Missoula for a vaccine development workshop with the CTM group. Thanks
for helping again with the samples!!

James and Jason:
Regarding the sample shipment, you can overnight them as a
lyophilized powder to:

University of Montana
Dept. of Chemistry &Biochemistry
c/o Bruce Bowler or Ariel Frederick
23 Campus Drive
Missoula, Montana 59812

Please weigh them out and indicate on your shipping list with what volume
they need to be suspended to get the desired concentrations. Our loading
volume will be 110 ul, and we would like to know the wavelength to use
where we would get 0.6 OD in a 3 mm pathlength centerpiece, with no more
than 0.3 OD of background absorbance from the buffer (when blanked against
water). We can use different wavelengths for each sample to optimize the
absorbance for each concentration.

The speed will be 60 krpm, so they should be measured in the An60Ti rotor,
at 20C.

Please ship so they will be available for pickup by us on Friday this week.
Please indicate the storage conditions - I assume for the lyophilized
powder 4C is adequate?

Thanks, -Borries
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