[Demelerlab] AUC Collaboration: Sizing Amyloid Beta derived Oligomers

[Jason Zhu]

Hi Bruce and Ariel,

A lower storage temperature (such as -20 oC) would be better however 4oC
should also work. Thanks for keeping an eye out for the shipment, I really
appreciate it!

Best,
Jason

On Wed, Jul 19, 2023 at 7:02 AM Bowler, Bruce <Bruce.Bowler at mso.umt.edu>
wrote:

> Hi Borries and Jason,
>
> We'll keep an eye out for your shipment. I take it you want it stored at 4
> oC when it arrives. Let me know if storage at a lower temperature is better.
>
> For pick up on Friday,  sometime between 10:30 am and 2:30 pm is best for
> me.
>
> Bruce
>
> Sent from my T-Mobile 5G Device
> Get Outlook for Android
> <https://urldefense.com/v3/__https://aka.ms/AAb9ysg__;!!CzAuKJ42GuquVTTmVmPViYEvSg!MMksFEt9h3wTNKbwVqKh2ZNZIsCFG0cn6YYIfTtveN0_nfAC7g4LrbIY6ui0RycSr9qsWB0CSNLYgYnRPYwi8Xt2He9g$>
> ------------------------------
> *From:* Jason Zhu <jasonz13 at uci.edu>
> *Sent:* Tuesday, July 18, 2023 8:57:55 PM
> *To:* Borries Demeler <demeler at gmail.com>
> *Cc:* James S. Nowick <jsnowick at uci.edu>; Henrickson, Amy <
> amy.henrickson at uleth.ca>; demelerlab at biophysics.uleth.ca <
> demelerlab at biophysics.uleth.ca>; maduni.ranasinghe at uleth.ca <
> maduni.ranasinghe at uleth.ca>; Bowler, Bruce <Bruce.Bowler at mso.umt.edu>;
> Frederick, Ariel <ariel.frederick at umconnect.umt.edu>
> *Subject:* Re: AUC Collaboration: Sizing Amyloid Beta derived Oligomers
>
> Hi Borries and James,
>
> I tried running different TFA concentrations on the nanodrop and got very
> noisy UV-Vis spectra that I don't fully trust. I enabled the automatic
> baseline correction and automatic pathlength adjustment settings on the
> nanodrop which seems to have helped with the negative absorbance values
> that don't make physical sense. I will meet with James tomorrow morning and
> will work out the details of getting accurate absorbance values for both
> TFA and F19Cha then.
>
> [image: image.png]
>
> I plan on weighing out the peptides, labeling them, and shipping them by
> the end of the day Wednesday (tomorrow). I'll send an update once we figure
> out the ideal wavelength for the different concentrations we plan to run at
> each concentration and when the peptide is on its way.
>
> For lyophilized powder, 4C should be adequate. F19Cha isn't prone to
> oxidizing from my experience working with it.
>
> Best,
> Jason
>
> On Tue, Jul 18, 2023 at 2:38 PM Borries Demeler <demeler at gmail.com> wrote:
>
> Hi Bruce and Ariel:
> We would like to have some samples shipped to U of Montana, and I would
> like to pick them up on Friday, if this is possible? The samples are
> lyophilized peptide samples and can probably be stored at 4C. I will be in
> Missoula on Friday  and Saturday, all day, our entire lab will actually be
> in Missoula for a vaccine development workshop with the CTM group. Thanks
> for helping again with the samples!!
>
> James and Jason:
> Regarding the sample shipment, you can overnight them as a
> lyophilized powder to:
>
> University of Montana
> Dept. of Chemistry &Biochemistry
> c/o Bruce Bowler or Ariel Frederick
> 23 Campus Drive
> Missoula, Montana 59812
>
> Please weigh them out and indicate on your shipping list with what volume
> they need to be suspended to get the desired concentrations. Our loading
> volume will be 110 ul, and we would like to know the wavelength to use
> where we would get 0.6 OD in a 3 mm pathlength centerpiece, with no more
> than 0.3 OD of background absorbance from the buffer (when blanked against
> water). We can use different wavelengths for each sample to optimize the
> absorbance for each concentration.
>
> The speed will be 60 krpm, so they should be measured in the An60Ti rotor,
> at 20C.
>
> Please ship so they will be available for pickup by us on Friday this
> week. Please indicate the storage conditions - I assume for the lyophilized
> powder 4C is adequate?
>
> Thanks, -Borries
>
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://www.biophysics.uleth.ca/pipermail/demelerlab/attachments/20230719/cb1fd80a/attachment-0001.html>
-------------- next part --------------
A non-text attachment was scrubbed...
Name: image.png
Type: image/png
Size: 38343 bytes
Desc: not available
URL: <http://www.biophysics.uleth.ca/pipermail/demelerlab/attachments/20230719/cb1fd80a/attachment-0001.png>