[Demelerlab] Update from the Barron lab

[Bird, Sophia]

Hello Josefine,

I am a lab tech working in the Demeler lab and will be helping organize instrument time and experimental planning for your project. Let's schedule a meeting to discuss the experimental design so we can get started. Please communicate your availability this week or next week and submit a new project in your LIMS account before the meeting so we can familiarize ourselves with it.

Let me know if you have any questions!

Best,

Sophia


________________________________
From: Demelerlab <demelerlab-bounces at biophysics.uleth.ca> on behalf of Josefine Eilsoe Nielsen <josefine at stanford.edu>
Sent: 05 November 2023 14:35
To: Borries Demeler <demeler at gmail.com>
Cc: demelerlab at biophysics.uleth.ca <demelerlab at biophysics.uleth.ca>; Annelise E. Barron <aebarron at stanford.edu>
Subject: Re: [Demelerlab] Update from the Barron lab


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So sorry to hear about your car, I am glad you were able to return to Leithbridge safe!
Samples should be ok in kitchen freezer, no worries.

Josefine

On 5 Nov 2023, at 13:19, Borries Demeler <demeler at gmail.com> wrote:


Hi Josefine,
my car developed engine issues on the way down to Missoula, so returned to Lethbridge and will have to get my car fixed first. I hope I can get it fixed reasonably fast. Is the sample ok in a kitchen freezer?
-Borries

On Thu, Nov 2, 2023 at 5:54 PM Josefine Eilsoe Nielsen <josefine at stanford.edu<mailto:josefine at stanford.edu>> wrote:
Hi,

That sounds like a good plan (sorry to hear about the instrument issues though). The peptides in powder form should be stored at at -20C for longer periods (we usually keep them at -80C, but Anaspec reports -20 as sufficient), but can be shipped at room temperature. Should we still ship them to Missoula?

Yes, I did check my LIMS account and could log in without issues, but I was having issues connecting using VNC, but I will try to download Ultrascan and connect my database as suggested. Will let you know if I run into any issues.

Best,
Josefine

31. okt. 2023 kl. 05:10 skrev Borries Demeler <demeler at gmail.com<mailto:demeler at gmail.com>>:

Hi Josefine,
I am glad to hear you guys figured out a way to prepare stable and colloidal systems of abeta and LL37,
I would be happy to help you with AUC to measure the LL37/abeta complex. Does ammonium hydroxide absorb in the low UV? If not, we can stick to low concentrations at low UV wavelengths and measure at low concentration. With a detailed dissolving protocol sending the powder should work fine. It may be a while before we can get to it since one of our machines is down right now. I would recommend sending the material to Missoula, since it is stable (it should be fine at 4C, correct?). Then I could pick it up the next time I am down here (driving back to Lethbridge today).

Regarding the UltraScan access, you can either install UltraScan on your system (https://www.ultrascan3.aucsolutions.com/download.php) and then connect it to your account in the CCH database ("Database login info" once you are logged in), or via VNC directly on our server. I could help you with either approach over Zoom if needed. It is possible we will have to recreate your account because inactive accounts get removed after a while. I checked, and your LIMS account with all of your data is still active and ready to go.

Let me know how you want to proceed.

Regards, -Borries

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