[Demelerlab] Spike Protein analysis

[Borries Demeler]

Hi Bao,
the limitation will be the signal we have from the labeled spike protein.
We can deal with the baseline offset from the free label, and the remaining
signal, while not a lot, should be sufficient to answer the composition
questions when we mix it with your lipids. Sophia is planning to run these
experiments at the end of the week, so we should have further results early
next week.

Best, -Borries

On Tue, Nov 21, 2023 at 10:06 AM Le, Bao <bao.le at mso.umt.edu> wrote:

> Thank you, Borries, for the update!
> I hope we can have some meaningful data for a better understanding of the
> vaccine mechanism and also for publication 🙂.
>
> Best,
> Bao
> ------------------------------
> *From:* Borries Demeler <demeler at gmail.com>
> *Sent:* Monday, November 20, 2023 2:48 PM
> *To:* demelerlab at biophysics.uleth.ca <demelerlab at biophysics.uleth.ca>;
> Le, Bao <bao.le at mso.umt.edu>; Le, Minh <Minh.Le at mso.umt.edu>
> *Subject:* Spike Protein analysis
>
> Hi Bao,
> Sophia measured your fluorescently labeled spike protein in our
> fluorescence instrument, and the signal was sufficient. However, it looks
> like most of the signal is coming from free fluorophore - which is OK,
> since we can eliminate it through data editing, but the total signal from
> your protein is not as clean as it could be if the labeling had been more
> efficient. Just so you know... here is a picture:
>
> [image: image.png]
> Sophia is planning to mix the remaining sample with the various lipids to
> see if there are any measurable interactions.
>
> Best wishes, -Borries
> Regards, -Borries
>
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