[Demelerlab] [External] Analytical Ultracentrifugation Analysis of Cannula-mimetic Proteins

[Conticello, Vincent]

Hi Reece. I will get back to you tomorrow AM. Best, Vince

Sent from my iPad

On Sep 6, 2023, at 7:11 PM, Martin, Reece <reece.martin at uleth.ca> wrote:


Hi Vince and Andres,

Considering that the samples arrived at room temperature (the exact length of time is unknown), and that an ice pack leaked (the samples appear to have been sealed properly). We are just looking to confirm that you feel it is worth continuing to process this batch of samples, taking into account your insight into the temperature-stability of the proteins at ambient temperature.

If so, as per the updated experimental design in the LIMS, we will start out running both CanA and Hyper2 at a high and low concentration in the EDTA-containing buffer at 20 degrees Celsius. To accomplish this, I need to know what buffer each of the protein stocks are in and whether or not they contain EDTA.

If this experiment is successful, we will repeat in the presence of calcium ions (10mM for CanA, and 1mM for Hyper2). For this we need to know at what temperature and for how long the samples should be incubated with the CaCl2 solution. Finally, if this experiment is successful, we will look at repeating with increased calcium ion concentrations, as well as running at a higher temperature such as 37 degrees Celsius.

I'll report back on the results from the first run, which will take place this week.

Cheers,

Reece

--
Reece Martin, B.Sc
M.Sc. Candidate
Northwest Biophysics Consortium
Alberta RNA Research and Training Institute (ARRTI)
Lab of Dr. Borries Demeler
Department of Chemistry and Biochemistry
University of Lethbridge
Email: reece.martin at uleth.ca
Phone: 403-498-5882
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