[Demelerlab] EXTERNAL: Gold nanoparticles

Tue Sep 19 12:58:00 MDT 2023

Perfect, thanks so much! -Borries

On Tue, Sep 19, 2023 at 12:36 PM Samantha R. Tracy <Samantha at clene.com>
wrote:

> Hi Borries,
>
> I have uploaded a TEM image that is representative of our nanoparticles.
> The expected particle size is 10-30 nanometers. Misha is going to get me an
> absorbance spectrum that I should hopefully be able to upload by tomorrow.
>
> Best,
>
> *Samantha Tracy*
> Research Engineer
> Clene Nanomedicine
>
> This email may contain material that is confidential, privileged, and/or
> attorney work product for the sole use of the intended recipient. Any
> unauthorized review, use, or distribution is prohibited and may be unlawful.
> ------------------------------
> *From:* Borries Demeler <demeler at gmail.com>
> *Sent:* Tuesday, September 19, 2023 11:57 AM
> *To:* Samantha R. Tracy <Samantha at clene.com>;
> demelerlab at biophysics.uleth.ca <demelerlab at biophysics.uleth.ca>;
> Harrington, Nick (Nicholas) J <NJHARRINGTON at beckman.com>
> *Subject:* Re: EXTERNAL: Gold nanoparticles
>
> Perfect, I reviewed the project request and I have most of the information
> I need to design the experiment. I was wondering if you could add a
> representative TEM image by uploading it in your LIMS account
> under "Supporting Files" (button in the left bar). You can associate it
> with your project request. Also, if you know the expected range of
> particle sizes in nanometers that would help as well to predict the most
> suitable rotor speed, and would save some experimentation time. If you
> happen to have an absorbance spectrum down to 220 nm or so of a very dilute
> sample, you can upload that as well.
>
> As soon as I have approval from Nick to proceed, Sophia in our lab will
> send you shipping instructions. Please include at least 15 ml of buffer for
> these samples for dilutions.
>
> Thanks! -Borries
>
>
>
> On Tue, Sep 19, 2023 at 8:27 AM Samantha R. Tracy <Samantha at clene.com>
> wrote:
>
> Hi Borries,
>
> We have just filled out the project details to the best of our ability. If
> you have any follow up questions let me know.
>
> Thanks,
>
> *Samantha Tracy*
> Research Engineer
> Clene Nanomedicine
>
> This email may contain material that is confidential, privileged, and/or
> attorney work product for the sole use of the intended recipient. Any
> unauthorized review, use, or distribution is prohibited and may be unlawful.
> ------------------------------
> *From:* Borries Demeler <demeler at gmail.com>
> *Sent:* Monday, September 18, 2023 5:51 PM
> *To:* Raj, Rekha M <RRAJ at beckman.com>; Harrington, Nick (Nicholas) J <
> NJHARRINGTON at beckman.com>; Samantha R. Tracy <Samantha at clene.com>;
> Mikhail Merzliakov <Mikhail at clene.com>
> *Cc:* Aysha Demeler <aysha.demeler at uleth.ca>; Elie Chdid <
> elie at aucsolutions.com>; Luc Roberts <luc at aucsolutions.com>
> *Subject:* EXTERNAL: Gold nanoparticles
>
> Hi everyone,
> as discussed today, in order to demonstrate the feasibility of the AUC
> method, and to contrast the results obtained from DLS, we plan to compare
> three samples of gold nanoparticles by sedimentation velocity. The goal is
> to demonstrate that AUC can pick up particle size distributions with much
> higher resolution than DLS and also can differentiate between different
> densities resulting from different metal core-hydration shell ratios.
>
> These questions have been previously evaluated by AUC in the following
> publications, two of which Aysha has contributed significantly already:
>
> <https://pubmed.ncbi.nlm.nih.gov/25010012/>
> Demeler B, Nguyen TL, Gorbet GE, Schirf V, Brookes EH, Mulvaney P,
> El-Ballouli AO, Pan J, Bakr OM, Demeler AK, Hernandez Uribe BI, Bhattarai
> N, Whetten RL. Characterization of size, anisotropy, and density
> heterogeneity of nanoparticles by sedimentation velocity. Anal Chem. 2014
> Aug 5;86(15):7688-95. doi: 10.1021/ac501722r. Epub 2014 Jul 18. PMID:
> 25010012; PMCID: PMC4144751. <https://pubmed.ncbi.nlm.nih.gov/25010012/>
>
> and:
>
> Gorbet G, Devlin T, Hernandez Uribe BI, Demeler AK, Lindsey ZL, Ganji S,
> Breton S, Weise-Cross L, Lafer EM, Brookes EH, Demeler B. A parametrically
> constrained optimization method for fitting sedimentation velocity
> experiments. Biophys J. 2014 Apr 15;106(8):1741-50. doi:
> 10.1016/j.bpj.2014.02.022. PMID: 24739173; PMCID: PMC4008825.
> <https://pubmed.ncbi.nlm.nih.gov/24739173/>
>
> Core-shell studies by analytical ultracentrifugation have been done by us
> as well:
>
> Walter J, Gorbet G, Akdas T, Segets D, Demeler B, Peukert W. 2D analysis
> of polydisperse core-shell nanoparticles using analytical
> ultracentrifugation. Analyst. 2016 Dec 19;142(1):206-217. doi:
> 10.1039/c6an02236g. PMID: 27934989; PMCID: PMC5167643.
> <https://pubmed.ncbi.nlm.nih.gov/27934989/>
>
> To proceed, we ask that the scientist in charge of this project creates a
> LIMS account in our LIMS server instance on this website:
>
> https://uslims.uleth.ca/uslims3_AUCCANADA/newaccount.php
>
> <https://uslims.uleth.ca/uslims3_AUCCANADA/newaccount.php>
> and create a project request ("Projects" button on the left bar) to get
> the ball rolling. Aysha will provide you with the shipping instructions and
> Luc or Elie will prepare a cost estimate for Nick to get a PO issued. We
> discussed obtaining ~2 ml of 1 OD at peak absorbance wavelength of three
> different samples to demonstrate that different answers can be obtained for
> each sample, and we may consider diluting the sample with the storage
> buffer (please provide 15 ml). Further details will be provided in the
> project request.
>
> Please let me know if you have any questions.
>
> Regards, -Borries
>
>
>
>
>
>
>
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> attachments, is intended solely for the use of the individual or entity
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> INFORMATION. If you are not the intended recipient, you are hereby notified
> that any use, dissemination, distribution, or copying of this electronic
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