[Demelerlab] EXTERNAL: Gold nanoparticles
Harrington, Nick (Nicholas) J
NJHARRINGTON at beckman.com
Tue Sep 26 11:19:55 MDT 2023
3 samples are fine with me. Please provide an updated quote.
Thanks,
Nick
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Confidential - Company Proprietary
From: Borries Demeler <demeler at gmail.com>
Sent: Monday, September 25, 2023 2:48 PM
To: Bird, Sophia <sophia.bird at uleth.ca>
Cc: Harrington, Nick (Nicholas) J <NJHARRINGTON at beckman.com>; Samantha R. Tracy <Samantha at clene.com>; demelerlab at biophysics.uleth.ca
Subject: Re: EXTERNAL: Gold nanoparticles
Hi Samantha, Sophia and Nick: I found a small correction - I believe we discussed three, not two, different samples to be compared to prove effectiveness of AUC on these samples. So please include all three :) Thanks, -Borries On Sun, Sep 24,
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Hi Samantha, Sophia and Nick:
I found a small correction - I believe we discussed three, not two, different samples to be compared to prove effectiveness of AUC on these samples.
So please include all three :)
Thanks, -Borries
On Sun, Sep 24, 2023 at 12:18:46AM +0000, Bird, Sophia wrote:
> Hello all,
>
> I am a student in Bo's lab and just wanted to provide some shipping information. To avoid any issues with customs, samples could be sent to the University of Montana and Bo can pick them up by this Thursday. Bruce Bowler can receive them for us, his contact information is below:
>
>
>
> University of Montana
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> 32 Campus Dr
>
> Missoula, MT 59812
>
> Attn: Bruce Bowler, Chemistry and Biochemistry (Chem 310)
>
> Phone: 406 243-6114
>
>
> I've attached a commercial invoice with the receiver information. Again, the experimental design was updated to outline the plan. Please ensure that 15 mL of buffer and the two 1.5 mL aliquots at 1 OD 525 nm are provided.
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> Let me know if there are any questions!
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>
> Thanks,
>
>
> Sophia
>
> ________________________________
> From: Demelerlab <demelerlab-bounces at biophysics.uleth.ca<mailto:demelerlab-bounces at biophysics.uleth.ca>> on behalf of Harrington, Nick (Nicholas) J <NJHARRINGTON at beckman.com<mailto:NJHARRINGTON at beckman.com>>
> Sent: 19 September 2023 10:33
> To: Borries Demeler <demeler at gmail.com<mailto:demeler at gmail.com>>; Samantha R. Tracy <Samantha at clene.com<mailto:Samantha at clene.com>>; demelerlab at biophysics.uleth.ca<mailto:demelerlab at biophysics.uleth.ca> <demelerlab at biophysics.uleth.ca<mailto:demelerlab at biophysics.uleth.ca>>
> Subject: Re: [Demelerlab] EXTERNAL: Gold nanoparticles
>
>
> Caution: This email was sent from someone outside of the University of Lethbridge. Do not click on links or open attachments unless you know they are safe. Suspicious emails should be forwarded to phishing at uleth.ca<mailto:phishing at uleth.ca>.
>
>
> Thanks Borries for leading the call yesterday and apologies to the group for my unexpected absence due to dental work and delays at their office. Ugh.
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>
>
>
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> The proposed experimentation, number of samples, and expected output is clear and fits with our expectations. Please have a quotation put together and sent to my attention for review. Once I have that, I will approve and we can move forward with sample shipping and subsequent analysis.
>
>
>
> Thanks!
>
> Nick
>
>
>
> Confidential - Company Proprietary
>
> From: Borries Demeler <demeler at gmail.com<mailto:demeler at gmail.com>>
> Sent: Tuesday, September 19, 2023 11:58 AM
> To: Samantha R. Tracy <Samantha at clene.com<mailto:Samantha at clene.com>>; demelerlab at biophysics.uleth.ca<mailto:demelerlab at biophysics.uleth.ca>; Harrington, Nick (Nicholas) J <NJHARRINGTON at beckman.com<mailto:NJHARRINGTON at beckman.com>>
> Subject: Re: EXTERNAL: Gold nanoparticles
>
>
>
> Perfect, I reviewed the project request and I have most of the information I need to design the experiment. I was wondering if you could add a representative TEM image by uploading it in your LIMS account under "Supporting Files"
>
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>
> Perfect, I reviewed the project request and I have most of the information I need to design the experiment. I was wondering if you could add a representative TEM image by uploading it in your LIMS account under "Supporting Files" (button in the left bar). You can associate it with your project request. Also, if you know the expected range of particle sizes in nanometers that would help as well to predict the most suitable rotor speed, and would save some experimentation time. If you happen to have an absorbance spectrum down to 220 nm or so of a very dilute sample, you can upload that as well.
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> As soon as I have approval from Nick to proceed, Sophia in our lab will send you shipping instructions. Please include at least 15 ml of buffer for these samples for dilutions.
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> Thanks! -Borries
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> On Tue, Sep 19, 2023 at 8:27 AM Samantha R. Tracy <Samantha at clene.com<mailto:Samantha at clene.com<mailto:Samantha at clene.com%3cmailto:Samantha at clene.com>>> wrote:
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> Hi Borries,
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> We have just filled out the project details to the best of our ability. If you have any follow up questions let me know.
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> Thanks,
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> Samantha Tracy
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> Research Engineer
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> Clene Nanomedicine
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>
>
> This email may contain material that is confidential, privileged, and/or attorney work product for the sole use of the intended recipient. Any unauthorized review, use, or distribution is prohibited and may be unlawful.
>
> ________________________________
>
> From: Borries Demeler <demeler at gmail.com<mailto:demeler at gmail.com<mailto:demeler at gmail.com%3cmailto:demeler at gmail.com>>>
> Sent: Monday, September 18, 2023 5:51 PM
> To: Raj, Rekha M <RRAJ at beckman.com<mailto:RRAJ at beckman.com<mailto:RRAJ at beckman.com%3cmailto:RRAJ at beckman.com>>>; Harrington, Nick (Nicholas) J <NJHARRINGTON at beckman.com<mailto:NJHARRINGTON at beckman.com<mailto:NJHARRINGTON at beckman.com%3cmailto:NJHARRINGTON at beckman.com>>>; Samantha R. Tracy <Samantha at clene.com<mailto:Samantha at clene.com<mailto:Samantha at clene.com%3cmailto:Samantha at clene.com>>>; Mikhail Merzliakov <Mikhail at clene.com<mailto:Mikhail at clene.com<mailto:Mikhail at clene.com%3cmailto:Mikhail at clene.com>>>
> Cc: Aysha Demeler <aysha.demeler at uleth.ca<mailto:aysha.demeler at uleth.ca<mailto:aysha.demeler at uleth.ca%3cmailto:aysha.demeler at uleth.ca>>>; Elie Chdid <elie at aucsolutions.com<mailto:elie at aucsolutions.com<mailto:elie at aucsolutions.com%3cmailto:elie at aucsolutions.com>>>; Luc Roberts <luc at aucsolutions.com<mailto:luc at aucsolutions.com<mailto:luc at aucsolutions.com%3cmailto:luc at aucsolutions.com>>>
> Subject: EXTERNAL: Gold nanoparticles
>
>
>
> Hi everyone,
>
> as discussed today, in order to demonstrate the feasibility of the AUC method, and to contrast the results obtained from DLS, we plan to compare three samples of gold nanoparticles by sedimentation velocity. The goal is to demonstrate that AUC can pick up particle size distributions with much higher resolution than DLS and also can differentiate between different densities resulting from different metal core-hydration shell ratios.
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> These questions have been previously evaluated by AUC in the following publications, two of which Aysha has contributed significantly already:
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> <https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=%3e%3e>
><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=%3e%3e>
> Demeler B, Nguyen TL, Gorbet GE, Schirf V, Brookes EH, Mulvaney P, El-Ballouli AO, Pan J, Bakr OM, Demeler AK, Hernandez Uribe BI, Bhattarai N, Whetten RL. Characterization of size, anisotropy, and density heterogeneity of nanoparticles by sedimentation velocity. Anal Chem. 2014 Aug 5;86(15):7688-95. doi: 10.1021/ac501722r. Epub 2014 Jul 18. PMID: 25010012; PMCID: PMC4144751.<https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=%3e%3e>
><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_25010012_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=Pr2sXjGYHcEs2Kh4yGtm-uv2zVtFIdk4iiUC26d7kgQ&e=%3e%3e>
>
>
> and:
>
>
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> Gorbet G, Devlin T, Hernandez Uribe BI, Demeler AK, Lindsey ZL, Ganji S, Breton S, Weise-Cross L, Lafer EM, Brookes EH, Demeler B. A parametrically constrained optimization method for fitting sedimentation velocity experiments. Biophys J. 2014 Apr 15;106(8):1741-50. doi: 10.1016/j.bpj.2014.02.022. PMID: 24739173; PMCID: PMC4008825.<https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_24739173_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=je35GYrI-3jZcUoJuixhZqJO35FJ0Y86rViw-Nz7HxY&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_24739173_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=je35GYrI-3jZcUoJuixhZqJO35FJ0Y86rViw-Nz7HxY&e=%3e%3e>
><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_24739173_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=je35GYrI-3jZcUoJuixhZqJO35FJ0Y86rViw-Nz7HxY&e=%3e%3e>
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> Core-shell studies by analytical ultracentrifugation have been done by us as well:
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>
> Walter J, Gorbet G, Akdas T, Segets D, Demeler B, Peukert W. 2D analysis of polydisperse core-shell nanoparticles using analytical ultracentrifugation. Analyst. 2016 Dec 19;142(1):206-217. doi: 10.1039/c6an02236g. PMID: 27934989; PMCID: PMC5167643.<https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_27934989_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=fWF0XIeLJLYPXBfDcwQZ581eJyQWsdQPfGmYGvXj8iw&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_27934989_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=fWF0XIeLJLYPXBfDcwQZ581eJyQWsdQPfGmYGvXj8iw&e=%3e%3e>
><https://urldefense.proofpoint.com/v2/url?u=https-3A__pubmed.ncbi.nlm.nih.gov_27934989_&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=fWF0XIeLJLYPXBfDcwQZ581eJyQWsdQPfGmYGvXj8iw&e=%3e%3e>
>
>
> To proceed, we ask that the scientist in charge of this project creates a LIMS account in our LIMS server instance on this website:
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>
>
> https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwIDaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=fHsQQ4-lq25VEM0aB9nl8LK9L-nqhPHnFx3eeZx4oyNYQR0kYlH6AT2lfRHp3_8x&s=V7Y_8KSTQs8lKUW2vuO1aA_ujowpCgvdQJU8Z8BU0tY&e=
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=%3e%3e>
><https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=%3e%3e>
>
> <https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=><https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=%3e%3e>
><https://urldefense.proofpoint.com/v2/url?u=https-3A__uslims.uleth.ca_uslims3-5FAUCCANADA_newaccount.php&d=DwMFaQ&c=9mghv0deYPYDGP-W745IEdQLV1kHpn4XJRvR6xMRXtA&r=ABy6E2p1lUDF_Nv0SrOTF281fkSdheTDlBdDP5aOa8w&m=0QAtnL0RCULIZuwls6omiHqL35tTkg-5LA8GuSl-Io_KnDO40lcsoXnQ_xeZEKwa&s=chs-OgiINwPtfAmyiyuPn-ZnMhtxf0fz8b9O0r1Tg3c&e=%3e%3e>
> and create a project request ("Projects" button on the left bar) to get the ball rolling. Aysha will provide you with the shipping instructions and Luc or Elie will prepare a cost estimate for Nick to get a PO issued. We discussed obtaining ~2 ml of 1 OD at peak absorbance wavelength of three different samples to demonstrate that different answers can be obtained for each sample, and we may consider diluting the sample with the storage buffer (please provide 15 ml). Further details will be provided in the project request.
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> Please let me know if you have any questions.
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> Regards, -Borries
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