[Demelerlab] AUC Collaboration: Sizing Amyloid Beta derived Oligomers

[Jason Zhu]

Hi Bruce, Ariel, and Borries,

The peptide (F19Cha) should ship tomorrow (Thursday) morning and arrive
before 10:30 am on Friday. I've attached the shipping label and
instructions for how much water to add to make 1, 3, and 9 mM samples.

Best,
Jason

On Wed, Jul 19, 2023 at 11:59 AM James S. Nowick <jsnowick at uci.edu> wrote:

> Hi All,
>
> The bottom line is that the absorbance of trifluoroacetate should be
> negligible with respect to the peptide. The peptide absorbs so strongly at
> 214 nm that you will need to go to longer wavelengths. Alternatively, I
> think interference optics might be a good option.
>
> Best,
> James
>
>
> On Jul 18, 2023, at 7:57 PM, Jason Zhu <jasonz13 at uci.edu> wrote:
>
> Hi Borries and James,
>
> I tried running different TFA concentrations on the nanodrop and got very
> noisy UV-Vis spectra that I don't fully trust. I enabled the automatic
> baseline correction and automatic pathlength adjustment settings on the
> nanodrop which seems to have helped with the negative absorbance values
> that don't make physical sense. I will meet with James tomorrow morning and
> will work out the details of getting accurate absorbance values for both
> TFA and F19Cha then.
>
> <image.png>
>
> I plan on weighing out the peptides, labeling them, and shipping them by
> the end of the day Wednesday (tomorrow). I'll send an update once we figure
> out the ideal wavelength for the different concentrations we plan to run at
> each concentration and when the peptide is on its way.
>
> For lyophilized powder, 4C should be adequate. F19Cha isn't prone to
> oxidizing from my experience working with it.
>
> Best,
> Jason
>
> On Tue, Jul 18, 2023 at 2:38 PM Borries Demeler <demeler at gmail.com> wrote:
>
>> Hi Bruce and Ariel:
>> We would like to have some samples shipped to U of Montana, and I would
>> like to pick them up on Friday, if this is possible? The samples are
>> lyophilized peptide samples and can probably be stored at 4C. I will be in
>> Missoula on Friday  and Saturday, all day, our entire lab will actually be
>> in Missoula for a vaccine development workshop with the CTM group. Thanks
>> for helping again with the samples!!
>>
>> James and Jason:
>> Regarding the sample shipment, you can overnight them as a
>> lyophilized powder to:
>>
>> University of Montana
>> Dept. of Chemistry &Biochemistry
>> c/o Bruce Bowler or Ariel Frederick
>> 23 Campus Drive
>> Missoula, Montana 59812
>>
>> Please weigh them out and indicate on your shipping list with what volume
>> they need to be suspended to get the desired concentrations. Our loading
>> volume will be 110 ul, and we would like to know the wavelength to use
>> where we would get 0.6 OD in a 3 mm pathlength centerpiece, with no more
>> than 0.3 OD of background absorbance from the buffer (when blanked against
>> water). We can use different wavelengths for each sample to optimize the
>> absorbance for each concentration.
>>
>> The speed will be 60 krpm, so they should be measured in the An60Ti
>> rotor, at 20C.
>>
>> Please ship so they will be available for pickup by us on Friday this
>> week. Please indicate the storage conditions - I assume for the lyophilized
>> powder 4C is adequate?
>>
>> Thanks, -Borries
>>
>
> James S. Nowick
> Distinguished Professor
> Department of Chemistry & Department of Pharmaceutical Sciences
>
> Department of Chemistry
> 4126 Natural Sciences 1
> University of California, Irvine
> Irvine, CA 92697-2025
>
> Phone:  (949)  824-6091
> e-mail: jsnowick at uci.edu
> Faculty Web Page: http://tinyurl.com/jsnowick/
> Research Group Web Page: http://tinyurl.com/nowickgroup/
> Pronouns: he/him/his
>
>
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