[Demelerlab] AUC Collaboration: Sizing Amyloid Beta derived Oligomers

[Borries Demeler]

Perfect! Thanks so much, and thanks to Bruce for handling our samples, I
will check in with you during the morning session of our workshop. Amy,
please remind me in case I forget.
Thanks, -Borries

On Wed, Jul 19, 2023 at 9:24 PM Jason Zhu <jasonz13 at uci.edu> wrote:

> Hi Bruce, Ariel, and Borries,
>
> The peptide (F19Cha) should ship tomorrow (Thursday) morning and arrive
> before 10:30 am on Friday. I've attached the shipping label and
> instructions for how much water to add to make 1, 3, and 9 mM samples.
>
> Best,
> Jason
>
> On Wed, Jul 19, 2023 at 11:59 AM James S. Nowick <jsnowick at uci.edu> wrote:
>
>> Hi All,
>>
>> The bottom line is that the absorbance of trifluoroacetate should be
>> negligible with respect to the peptide. The peptide absorbs so strongly at
>> 214 nm that you will need to go to longer wavelengths. Alternatively, I
>> think interference optics might be a good option.
>>
>> Best,
>> James
>>
>>
>> On Jul 18, 2023, at 7:57 PM, Jason Zhu <jasonz13 at uci.edu> wrote:
>>
>> Hi Borries and James,
>>
>> I tried running different TFA concentrations on the nanodrop and got very
>> noisy UV-Vis spectra that I don't fully trust. I enabled the automatic
>> baseline correction and automatic pathlength adjustment settings on the
>> nanodrop which seems to have helped with the negative absorbance values
>> that don't make physical sense. I will meet with James tomorrow morning and
>> will work out the details of getting accurate absorbance values for both
>> TFA and F19Cha then.
>>
>> <image.png>
>>
>> I plan on weighing out the peptides, labeling them, and shipping them by
>> the end of the day Wednesday (tomorrow). I'll send an update once we figure
>> out the ideal wavelength for the different concentrations we plan to run at
>> each concentration and when the peptide is on its way.
>>
>> For lyophilized powder, 4C should be adequate. F19Cha isn't prone to
>> oxidizing from my experience working with it.
>>
>> Best,
>> Jason
>>
>> On Tue, Jul 18, 2023 at 2:38 PM Borries Demeler <demeler at gmail.com>
>> wrote:
>>
>>> Hi Bruce and Ariel:
>>> We would like to have some samples shipped to U of Montana, and I would
>>> like to pick them up on Friday, if this is possible? The samples are
>>> lyophilized peptide samples and can probably be stored at 4C. I will be in
>>> Missoula on Friday  and Saturday, all day, our entire lab will actually be
>>> in Missoula for a vaccine development workshop with the CTM group. Thanks
>>> for helping again with the samples!!
>>>
>>> James and Jason:
>>> Regarding the sample shipment, you can overnight them as a
>>> lyophilized powder to:
>>>
>>> University of Montana
>>> Dept. of Chemistry &Biochemistry
>>> c/o Bruce Bowler or Ariel Frederick
>>> 23 Campus Drive
>>> Missoula, Montana 59812
>>>
>>> Please weigh them out and indicate on your shipping list with what
>>> volume they need to be suspended to get the desired concentrations. Our
>>> loading volume will be 110 ul, and we would like to know the wavelength to
>>> use where we would get 0.6 OD in a 3 mm pathlength centerpiece, with no
>>> more than 0.3 OD of background absorbance from the buffer (when blanked
>>> against water). We can use different wavelengths for each sample to
>>> optimize the absorbance for each concentration.
>>>
>>> The speed will be 60 krpm, so they should be measured in the An60Ti
>>> rotor, at 20C.
>>>
>>> Please ship so they will be available for pickup by us on Friday this
>>> week. Please indicate the storage conditions - I assume for the lyophilized
>>> powder 4C is adequate?
>>>
>>> Thanks, -Borries
>>>
>>
>> James S. Nowick
>> Distinguished Professor
>> Department of Chemistry & Department of Pharmaceutical Sciences
>>
>> Department of Chemistry
>> 4126 Natural Sciences 1
>> University of California, Irvine
>> Irvine, CA 92697-2025
>>
>> Phone:  (949)  824-6091
>> e-mail: jsnowick at uci.edu
>> Faculty Web Page: http://tinyurl.com/jsnowick/
>> Research Group Web Page: http://tinyurl.com/nowickgroup/
>> Pronouns: he/him/his
>>
>>
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