[Demelerlab] Update from the Barron lab

[Borries Demeler]

Hi Josefine,
my car developed engine issues on the way down to Missoula, so returned to
Lethbridge and will have to get my car fixed first. I hope I can get it
fixed reasonably fast. Is the sample ok in a kitchen freezer?
-Borries

On Thu, Nov 2, 2023 at 5:54 PM Josefine Eilsoe Nielsen <
josefine at stanford.edu> wrote:

> Hi,
>
> That sounds like a good plan (sorry to hear about the instrument issues
> though). The peptides in powder form should be stored at at -20C for longer
> periods (we usually keep them at -80C, but Anaspec reports -20 as
> sufficient), but can be shipped at room temperature. Should we still ship
> them to Missoula?
>
> Yes, I did check my LIMS account and could log in without issues, but I
> was having issues connecting using VNC, but I will try to download
> Ultrascan and connect my database as suggested. Will let you know if I run
> into any issues.
>
> Best,
> Josefine
>
> 31. okt. 2023 kl. 05:10 skrev Borries Demeler <demeler at gmail.com>:
>
> Hi Josefine,
> I am glad to hear you guys figured out a way to prepare stable and
> colloidal systems of abeta and LL37,
> I would be happy to help you with AUC to measure the LL37/abeta complex.
> Does ammonium hydroxide absorb in the low UV? If not, we can stick to low
> concentrations at low UV wavelengths and measure at low concentration. With
> a detailed dissolving protocol sending the powder should work fine. It may
> be a while before we can get to it since one of our machines is down right
> now. I would recommend sending the material to Missoula, since it is stable
> (it should be fine at 4C, correct?). Then I could pick it up the next time
> I am down here (driving back to Lethbridge today).
>
> Regarding the UltraScan access, you can either install UltraScan on your
> system (https://www.ultrascan3.aucsolutions.com/download.php) and then
> connect it to your account in the CCH database ("Database login info" once
> you are logged in), or via VNC directly on our server. I could help you
> with either approach over Zoom if needed. It is possible we will have to
> recreate your account because inactive accounts get removed after a while.
> I checked, and your LIMS account with all of your data is still active and
> ready to go.
>
> Let me know how you want to proceed.
>
> Regards, -Borries
>
>
>
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