[Demelerlab] AUC project preps

[Borries Demeler]

Hi Vince and Winston,
thanks for taking the time to explain your project to us today.  We have a
good idea of what needs to be done here, and the AUC experiments should be
straight-forward (famous last words).

To get the ball rolling, I would like for Winston to set up a LIMS account
at:  https://uslims.uleth.ca/uslims3_CCH/newaccount.php

Once you create your account, you will get a temporary password in your
e-mail, and you can log into your account and change the password using the
"Change my Info" button in the left bar to something that is more memorable
than the random password that was sent to you. Next, you should submit a
project request by clicking on the "Projects" button in the left bar, and
clicking on "New" in the project list. There are a number of questions to
be answered and you can simply fill out as much as you can, leaving the
"Experimental Design" section blank for us to fill out. The rest of the
questions should have obvious answers for you. Here, please include
information about the sample matrix you would like to examine by AUC
(provide your optimal wishlist, assuming cost is no issue), and indicate
the concentration you would like to measure for each sample. If you want to
measure mass action, we should consider running multiple concentrations (at
least two). Please include storage conditions.

Finally, please upload some supporting info in the "Supporting Files"
button in the left bar. There, you can upload a plot of the buffer at the
buffer concentration you plan to use (blanked against water), and an
absorbance profile for each sample you would like to ship at a
concentration of 1 OD or less at the wavelength that absorbs the most in
the wavelength range where you buffer has less than 0.4 OD background. This
would typically be the lowest wavelength that is included. This will assure
that we stay in the dynamic range of our detector, and get a linear
response when we perform the experiments in absorbance mode. The peptide
spectra should be measured using a spectrophotometer blanked against the
buffer in which they are dissolved, and indicate the peptide concentrations
at which they were measured (although if they have a trp residue, we can
also determine this later from the 280 nm absorbance).

Once I have these details together, we will discuss your project internally
and edit your project request to add the experimental design. At this point
we should also know the volume and concentration we will need you to send
to Lethbridge. Sophia will send you shipping and pre-clearance
instructions, and please include sufficient buffer volume for us to make
dilutions (if needed). We prefer to make the dilutions ourselves, since we
have to pay import tax and customs brokerage fees for each sample. Please
list the value of samples at $1/tube, this keeps our costs lower.

When you reply to this message, or send a message to
demelerlab at biophysics.uleth.ca, please keep in mind that your e-mail
message will initially be quarantined. I will get a notice and will release
it from quarantine.

Looking forward to working again with your group, Vince!
best wishes, -Borries
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