[Demelerlab] More sedimentation?

[Borries Demeler]

Hello Richard,
I think the comparison of such molecules would be a very reasonable test to
do by AUC. How much we will learn will depend on the size of the bound
molecules, and their effect on the folding state or oligomerization state.
Shape changes could have no effect if the overall anisotropy doesn't change
with the shape change, or be too subtle to be detected.

Since I am not totally clear what is being measured, and what the questions
are, I think it would be helpful to have a zoom meeting and to go over the
details. Potentially, multi-wavelength AUC could help with the
hetero-association between the molecules.

If you want to set up a zoom meeting, please send me your availability for
next week.

Regards, -Borries

On Wed, Sep 20, 2023 at 3:01 PM Richard Willson <rcwwww at gmail.com> wrote:

> *EXTERNAL EMAIL*
>
> Dear Borries, we have found some interesting molecules that bind to
> antibodies, and we were wondering if you might be free to do a fairly
> simple characterization of them. These would be single domains of the three
> domain object that you worked on before. I am wondering if it is realistic
> to compare sedimentation rates as evidence of the comparability or
> dissimilarity of overall folded confirmation? Is that a reasonable kind of
> interpretation to apply to these data?
>
> Thanks!
>
> Best regards,
>
> Richard
>
> CAUTION: This message originated outside of UT Health San Antonio. Please
> exercise caution when clicking on links or opening attachments.
>
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://www.biophysics.uleth.ca/pipermail/demelerlab/attachments/20230920/01db2a01/attachment.html>