[Demelerlab] FedEx shipment

[Baranwal, Jyoti]

Dear Dr. Demeler,
I would suggest storing the protein at -80 degree C for long term. Usually, I have seen this protein is stable, whenever I have worked with it. If it is possible, I would suggest keeping it on dry ice while traveling to Canada. Because I know it doesn't precipitate at 4C but because we want to check the oligomerization state of this protein. I have no idea what will happen when we freeze thaw from -80 to 4, and then back to -80.


Thank you!

With best regards,

Jyoti

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________________________________
From: Borries Demeler <demeler at gmail.com>
Sent: Thursday, December 28, 2023 2:17:27 PM
To: Baranwal, Jyoti <baranwal at uthscsa.edu>
Cc: Gupta, Yogesh K <GuptaY at uthscsa.edu>; demelerlab at biophysics.uleth.ca <demelerlab at biophysics.uleth.ca>
Subject: Re: Fw: FedEx shipment

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Great, thank you Jyoti! That should be more than sufficient to do all of the experiments. When I get back to Lethbridge we will schedule a zoom call with the rest of the lab so everyone is on the same page, and then we can discuss further details and confirm our experimental designs. One more question: is the sample stable at 4C, or must it be stored at -80 or -20C? Can it be at 4C (on ice) while driving back to Canada - about 6 hours?
Thanks, -Borries
On Thu, Dec 28, 2023, 12:59 Baranwal, Jyoti <baranwal at uthscsa.edu<mailto:baranwal at uthscsa.edu>> wrote:
Dear Dr. Demeler,
I have packed the sample and have sent it by overnight FedEx. Most probably it will reach you by tomorrow. It contains 13 vials of 100ul, 13 mg/ml of protein (hope that is sufficient to try all different concentrations and buffers)  and 10ml of 8 different buffers listed below.

  1.  200 mM LiSO4_25 mM Sodium acetate pH 4.5
  2.  15mM Tris-HCl_50mM KCl pH7.5
  3.  1x PBS
  4.  15mM Sodium acetate_100 mM NaCl pH 4.5
  5.  25mM MES_100mM NaCl pH 5.5
  6.  25mM MES_100mM NaCl pH 6.5
  7.  15mM Tris-HCl_100mM NaCl pH 7.5
  8.  15mM Tris-HCl_100mM NaCl pH 8.5


I have attached the FedEx tracking label with this email for your reference.

My sincere thanks to you!


Jyoti Baranwal


Postdoctoral Researcher
Gupta Lab
Greehey Children's Cancer Research Institute (GCCRI)
University of Texas Health Science Center at San Antonio
Email: baranwal at uthscsa.edu<mailto:baranwal at uthscsa.edu>

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