[Demelerlab] EXTERNAL: Gold nanoparticles

[Borries Demeler]

Perfect, I reviewed the project request and I have most of the information
I need to design the experiment. I was wondering if you could add a
representative TEM image by uploading it in your LIMS account
under "Supporting Files" (button in the left bar). You can associate it
with your project request. Also, if you know the expected range of
particle sizes in nanometers that would help as well to predict the most
suitable rotor speed, and would save some experimentation time. If you
happen to have an absorbance spectrum down to 220 nm or so of a very dilute
sample, you can upload that as well.

As soon as I have approval from Nick to proceed, Sophia in our lab will
send you shipping instructions. Please include at least 15 ml of buffer for
these samples for dilutions.

Thanks! -Borries



On Tue, Sep 19, 2023 at 8:27 AM Samantha R. Tracy <Samantha at clene.com>
wrote:

> Hi Borries,
>
> We have just filled out the project details to the best of our ability. If
> you have any follow up questions let me know.
>
> Thanks,
>
> *Samantha Tracy*
> Research Engineer
> Clene Nanomedicine
>
> This email may contain material that is confidential, privileged, and/or
> attorney work product for the sole use of the intended recipient. Any
> unauthorized review, use, or distribution is prohibited and may be unlawful.
> ------------------------------
> *From:* Borries Demeler <demeler at gmail.com>
> *Sent:* Monday, September 18, 2023 5:51 PM
> *To:* Raj, Rekha M <RRAJ at beckman.com>; Harrington, Nick (Nicholas) J <
> NJHARRINGTON at beckman.com>; Samantha R. Tracy <Samantha at clene.com>;
> Mikhail Merzliakov <Mikhail at clene.com>
> *Cc:* Aysha Demeler <aysha.demeler at uleth.ca>; Elie Chdid <
> elie at aucsolutions.com>; Luc Roberts <luc at aucsolutions.com>
> *Subject:* EXTERNAL: Gold nanoparticles
>
> Hi everyone,
> as discussed today, in order to demonstrate the feasibility of the AUC
> method, and to contrast the results obtained from DLS, we plan to compare
> three samples of gold nanoparticles by sedimentation velocity. The goal is
> to demonstrate that AUC can pick up particle size distributions with much
> higher resolution than DLS and also can differentiate between different
> densities resulting from different metal core-hydration shell ratios.
>
> These questions have been previously evaluated by AUC in the following
> publications, two of which Aysha has contributed significantly already:
>
> <https://pubmed.ncbi.nlm.nih.gov/25010012/>
> Demeler B, Nguyen TL, Gorbet GE, Schirf V, Brookes EH, Mulvaney P,
> El-Ballouli AO, Pan J, Bakr OM, Demeler AK, Hernandez Uribe BI, Bhattarai
> N, Whetten RL. Characterization of size, anisotropy, and density
> heterogeneity of nanoparticles by sedimentation velocity. Anal Chem. 2014
> Aug 5;86(15):7688-95. doi: 10.1021/ac501722r. Epub 2014 Jul 18. PMID:
> 25010012; PMCID: PMC4144751. <https://pubmed.ncbi.nlm.nih.gov/25010012/>
>
> and:
>
> Gorbet G, Devlin T, Hernandez Uribe BI, Demeler AK, Lindsey ZL, Ganji S,
> Breton S, Weise-Cross L, Lafer EM, Brookes EH, Demeler B. A parametrically
> constrained optimization method for fitting sedimentation velocity
> experiments. Biophys J. 2014 Apr 15;106(8):1741-50. doi:
> 10.1016/j.bpj.2014.02.022. PMID: 24739173; PMCID: PMC4008825.
> <https://pubmed.ncbi.nlm.nih.gov/24739173/>
>
> Core-shell studies by analytical ultracentrifugation have been done by us
> as well:
>
> Walter J, Gorbet G, Akdas T, Segets D, Demeler B, Peukert W. 2D analysis
> of polydisperse core-shell nanoparticles using analytical
> ultracentrifugation. Analyst. 2016 Dec 19;142(1):206-217. doi:
> 10.1039/c6an02236g. PMID: 27934989; PMCID: PMC5167643.
> <https://pubmed.ncbi.nlm.nih.gov/27934989/>
>
> To proceed, we ask that the scientist in charge of this project creates a
> LIMS account in our LIMS server instance on this website:
>
> https://uslims.uleth.ca/uslims3_AUCCANADA/newaccount.php
>
> <https://uslims.uleth.ca/uslims3_AUCCANADA/newaccount.php>
> and create a project request ("Projects" button on the left bar) to get
> the ball rolling. Aysha will provide you with the shipping instructions and
> Luc or Elie will prepare a cost estimate for Nick to get a PO issued. We
> discussed obtaining ~2 ml of 1 OD at peak absorbance wavelength of three
> different samples to demonstrate that different answers can be obtained for
> each sample, and we may consider diluting the sample with the storage
> buffer (please provide 15 ml). Further details will be provided in the
> project request.
>
> Please let me know if you have any questions.
>
> Regards, -Borries
>
>
>
>
>
>
>
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